Genome-Wide Identification of Circular RNAs in Fungal Parasite Nosema ceranae

文献类型： 外文期刊

第一作者： Guo, Rui

作者： Guo, Rui;Chen, Dafu;Chen, Huazhi;Xiong, Cuiling;Zheng, Yanzhen;Du, Yu;Geng, Sihai;Wang, Haipeng;Zhou Dingding;Guo Yilong;Hou, Chunsheng

作者机构：

期刊名称：CURRENT MICROBIOLOGY （ 影响因子：2.188； 五年影响因子：2.197 ）

ISSN： 0343-8651

年卷期： 2018 年 75 卷 12 期

页码：

收录情况： SCI

摘要： Circular RNAs (circRNAs) are newly discovered endogenous non-coding RNAs (ncRNAs) that play key roles in microRNA function and transcriptional regulation. Though a large number of circRNAs had been identified in animals and plants, however, little is known regarding circRNAs in Nosema ceranae, a widespread fungal parasite of honeybee. In this study, using deep sequencing technology and bioinformatic analysis, we predicted 204 circRNAs from N. ceranae spore samples, including 174 exonic circRNAs and 30 intergenic circRNAs. In addition, the expression of seven N. ceranae circRNAs was confirmed by RT-PCR assay. Furthermore, regulation networks of circRNAs were constructed, and 15 circRNAs were found to act as sponges of the corresponding three miRNAs. GO categorization and pathway enrichment analysis suggested that the circRNAs are likely to play significant roles in N. ceranae spore. This is the first report of circRNAs generated by a microsporidia species. Our results provide novel insights into understanding the basic biology of N. ceranae.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序