文献类型： 外文期刊
第一作者： Xie, Shi-Chen
作者： Xie, Shi-Chen;Jiang, Meng-Meng;Sheng, Jin-Liang;Xie, Shi-Chen;Zou, Yang;Chen, Dan;Yuan, Xiao-Dan;Zhu, Xing-Quan;Li, Zhao;Zou, Feng-Cai;Yang, Jian-Fa;Zhu, Xing-Quan
作者机构：
期刊名称：BIOMED RESEARCH INTERNATIONAL （ 影响因子：3.411； 五年影响因子：3.62 ）
ISSN： 2314-6133
年卷期： 2018 年
页码：
收录情况： SCI
摘要： Giardia duodenalis is an important zoonotic parasite which can parasitize in the intestines of humans and various animals. However, the information about the prevalence and genetic diversity of G. duodenalis in goats in China is limited. It is yet to be known whether Yunnan black goats, a unique goat breed in subtropical Yunnan province, southwestern China, are infected with G. duodenalis. Thus, a total of 907 fecal samples were collected from Yunnan black goats in five regions in Yunnan province, to estimate the prevalence and genotypes of G. duodenalis using a PCR-based approach. The G. duodenalis prevalence is 4.2% (38/907) in Yunnan black goats by nested amplification of the beta-giardin (bg) gene, and the genotypes are identified as assemblage E, with 5 novel subtypes (E-11-E-15). Multilocus sequence typing revealed that 11, 18, and 38 samples were amplifiable on tpi (triose phosphate isomerase), gdh (glutamate dehydrogenase), and bg locus, respectively, and identified three novel multilocus genotypes (M LG s): MLGE9-MLGE11. To our knowledge, this is the first report of G. duodenalis prevalence and genotypes in Yunnan black goats in China, which extended the host range of G. duodenalis and provided basic data for controlling G. duodenalis infection in Yunnan black goats.
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