Cry78Aa, a novel Bacillus thuringiensis insecticidal protein with activity against Laodelphax striatellus and Nilaparvata lugens

文献类型： 外文期刊

第一作者： Gao, Jiguo

作者： Gao, Jiguo;Wang, Yinglong;Liu, Yonglei;Zhang, Jie;Song, Fuping;Shu, Changlong;Liu, Yonglei;Crickmore, Neil

作者机构：

关键词： Planthopper; Hemiptera; Insecticidal protein

期刊名称：JOURNAL OF INVERTEBRATE PATHOLOGY （ 影响因子：2.841； 五年影响因子：3.368 ）

ISSN： 0022-2011

年卷期： 2018 年 158 卷

页码：

收录情况： SCI

摘要： Transgenic plants expressing insecticidal proteins originating from Bacillus thuringiensis (Bt) have successfully been used to control lepidopteran and coleopteran pests with chewing mouthparts. However, only a handful of Bt proteins have been identified that have bioactivity against sap sucking pests (Hemiptera), including aphids, whiteflies, plant bugs and planthoppers. A novel Bt insecticidal protein with significant toxicity against a hemipteran insect pest is described here. The gene encoding the 359 amino acid, 40.7 kDa protein was cloned from strain C9F1. After expression and purification of the toxin, its median lethal concentration (LC50) values against Laodelphax striatellus and Nilaparvata lugens were determined as 6.89 mu g/mL and 15.78 mu g/mL respectively. Analysis of the toxin sequence revealed the presence of both Toxin_10 and Ricin_B_Lectin domains.

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