Isolation and biological characteristics of multipotent mesenchymal stromal cells derived from chick embryo intestine
文献类型： 外文期刊
第一作者： Wang, J. J.
作者： Wang, J. J.;Zhang, W. X.;Wang, K. F.;Han, X.;Guan, W. J.;Ma, Y. H.;Wang, J. J.;Zhang, S.
作者机构：
关键词： Characterisation; chick embryo; mesenchymal stromal cells; intestinal stem cells; multi-differentiation
期刊名称：BRITISH POULTRY SCIENCE （ 影响因子：2.095； 五年影响因子：2.266 ）
ISSN： 0007-1668
年卷期： 2018 年 59 卷 5 期
页码：
收录情况： SCI
摘要： 1. Over the past decade, rapid advancement in isolation methods for identifying markers of the once elusive intestinal stem cell (ISC) populations has laid the foundation for unravelling their complex interrelationships during homeostasis. Study on ISC in avian intestinal tissue might play a pivotal foundation for further studies on the epithelial-to-mesenchymal transition (EMT) in gastrointestinal disease and cell-based therapy as well as intestinal tissue engineering.2. The following experiment isolated a population of fibroblast-like, plastic adhering cells derived from chick embryo intestine, showing a strong self-renewing and proliferative ability, which was maintained in vitro up to passage 25. The findings included growth characteristics, detected expression of cell surface markers and characterised the capability of these cells to differentiate towards the osteogenic, adipogenic, and chondrogenic cell lineages.3. RT-PCR analysis showed that these cells from chick embryos expressed mesenchymal stromal cell markers CD44, CD90 and VIMENTIN as well as ISC-specific genes LGR5, MI1, SMOC2, BMI1, and HOPX. Immunofluorescence and flow cytometry confirmed this biology characterisation further.4. In conclusion, cells were isolated from the intestine of 18-day-old chicken embryos that exhibited the biological characteristics of mesenchymal stromal cells as well as markers of intestinal stem cells. Our findings may provide a novel insight for in vitro cell culture and characteristics of ISCs in avian species, which may also indicate a benefit for obtaining cell source for intestinal tissue engineering as well as cell-based investigation for gastrointestinal disease and treatment.
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