Identification and immunogenic evaluation of T cell epitopes based on tembusu virus envelope protein in ducks

文献类型： 外文期刊

第一作者： Zhao, Dongmin

作者： Zhao, Dongmin;Han, Kaikai;Zhang, Lijiao;Wang, Huili;Tian, Yujie;Huang, Xinmei;Liu, Qingtao;Yang, Jing;Liu, Yuzhuo;Li, Yin;Zhao, Dongmin;Han, Kaikai;Zhang, Lijiao;Wang, Huili;Tian, Yujie;Huang, Xinmei;Liu, Qingtao;Yang, Jing;Liu, Yuzhuo;Li, Yin

作者机构：

关键词： Tembusu virus; T cell epitope; Cell-mediate immune response; DNA vaccine

期刊名称：VIRUS RESEARCH （ 影响因子：3.303； 五年影响因子：3.445 ）

ISSN： 0168-1702

年卷期： 2018 年 257 卷

页码：

收录情况： SCI

摘要： Newly emerging tembusu virus (TMUV) is a severe threat to poultry industry and causes huge economic losses. Humoral and cell-mediated immunity are both play vital roles in TMUV infection. Up to now, there has been no report on identification of T cell epitopes of the TMUV. In this work, we identified T cell epitopes within TMUV envelope (E) protein using synthesized peptides predicted in silico. A total of ten peptides could stimulate TMUV-specific T cells in murine ELISPOT and duck lymphocyte proliferation assay. Subsequently, DNA vaccine containing these T cell epitopes was constructed (pVAX-T) and the expression of multiepitope protein was confirmed by transfection of BHK-21 cells in vitro. Ducks were administrated intramusclarly to evaluated the immunologic effect of pVAX-T. In ducks immunized with pVAX-T, antibody against TMUV was undetectable, but the expression level of cytokines (IL-2, IL-6, IFN-gamma) was upregulated both in peripheral blood lymphocytes and spleen. Furthermore, TMUV challenge revealed that cell-mediated immune response sitmulated by pVAX-T contributed to protection against TMUV infection. The identification of these T cell epitopes will contribute to designing epitope vaccine for preventing infection of TMUV and possibly provide the basis for further studies on cell mediate immune response activated by TMUV.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序