High glucose affected respiratory burst activity of peripheral leukocyte via G6PD and NOX inhibition in Megalobrama amblycephala

文献类型： 外文期刊

第一作者： Huang, Xin

作者： Huang, Xin;Pan, Wen-Jing;Ge, Xian-Ping;Liu, Bo;Miao, Ling-Hong;Lin, Yan;Ren, Ming-Chun;Ge, Xian-Ping;Liu, Bo;Zhou, Qun-Lan

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关键词： Megalobrama amblycephala; Leukocyte; G6PD; Respiratory burst; Glucose

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.581； 五年影响因子：4.851 ）

ISSN： 1050-4648

年卷期： 2018 年 83 卷

页码：

收录情况： SCI

摘要： High glucose levels are known to impair growth and immune function in fish. Here we investigated the role of glucose-6-phosphate dehydrogenase (G6PD) and NADPH oxidase (NOX) in high glucose-associated impairment of leukocyte respiratory burst activity in Megalobrama amblycephala. We cultured peripheral leukocytes isolated from M. amblycephala with media containing no glucose (non-glucose group), 11.1 mmol/L D-glucose (physiologic glucose group), 22.2 mmol/L D-glucose (high-glucose group), or 11.1 mmol/L D-glucose + 100 mu mol/L dehydroepiandrosterone (DHEA) (DHEA-treated group). After 24 h, we assayed production of reactive oxygen species (ROS) as a measure of respiratory burst function as well as activity of G6PD and NOX. The high-glucose group and DHEA-treated group showed significantly reduced respiratory burst function, reduced production of ROS, and reduced G6PD and NOX activity at 24 h, compared to the non-glucose and physiologic glucose groups (P < 0.05). The degree of impairment was similar between high-glucose and DHEA-treated groups (P > 0.05). These findings suggest that reduced NADPH availability likely underlies the suppression of respiratory burst function in M. amblycephala leukocytes exposed to high glucose levels.

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