Thermally controlled biotransformation of glycyrrhizic acid via an asymmetric temperature-responsive polyurethane membrane

文献类型： 外文期刊

第一作者： Wu, Xiuhong

作者： Wu, Xiuhong;Wang, Shaoyan;Zhang, Lina;Wu, Lidong;Chen, Yi;Wu, Xiuhong;Wu, Lidong;Chen, Yi

作者机构：

期刊名称：RSC ADVANCES （ 影响因子：3.361； 五年影响因子：3.39 ）

ISSN： 2046-2069

年卷期： 2018 年 8 卷 61 期

页码：

收录情况： SCI

摘要： Separating a target product from a relatively complex bioreaction system is often difficult. In this work, a smart bioreaction system was developed by using the special characteristic of temperature-responsive polyurethane (TRPU). By combining solvent evaporation with a wet phase inversion technique, an asymmetric membrane consisting of an integral and dense skin layer supported by a porous sublayer was prepared from a thermally responsive polyurethane that experiences a sudden free volume increase upon heating through a phase transition temperature of 56 degrees C. Subsequently, the asymmetric TRPU membrane served as the carrier of an immobilized enzyme, wherein -glucuronidase was multipoint-conjugated by using biotin and streptavidin on the porous sublayer. Then, the material-asymmetric TRPU membrane served jointly as the antennae as well as the actuator, which reversibly responds to temperature to switch (on-off) the access of the reactant glycyrrhizic acid (GL). Under the optimal temperature (40 degrees C) and pH (7.0) conditions, the immobilized -glucuronidase contributed to almost 33% yield of glycyrrhetinic acid 3-O-mono--d-glucuronide (GAMG) of the isolated counterpart for the same concentration of substrate (250 mg L-1) reaction for 24 h, while costing 1% of that of the isolated -glucuronidase. Kinetic results showed that V-max and K-m values were 8.89 x 10(3) mg L-1 and 2.30 x 10(3) mg L-1 h(-1), respectively. The specific functional polymer-immobilized -glucuronidase design serves as a bioreactor of GL into GAMG, as well as a separator deliberately irritated and controlled by temperature. This smart support material presents a potential facilitator for the separation of complex biotransformation reactions.

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