Venom from the scorpion Heterometrus liangi inhibits HeLa cell proliferation by inducing p21 expression

文献类型： 外文期刊

第一作者： Wang, Juan

作者： Wang, Juan;Wang, Zhi;Wang, Juan;Peng, Yuande;Wang, Zhi;Chai, Xiangping;Lv, Zhiyue;Song, Qisheng

作者机构：

关键词： Heterometrus liangi; Crude venom; HeLa cells; Cell proliferation; Apoptosis; p21 protein

期刊名称：BIOLOGIA （ 影响因子：1.35； 五年影响因子：1.139 ）

ISSN： 0006-3088

年卷期： 2018 年 73 卷 11 期

页码：

收录情况： SCI

摘要： Scorpion venoms are important sources for identifying and developing promising biomolecules for cancer treatment. To search for promising biomolecules for cancer research, the effects of crude venom from the scorpion Heterometrus liangi (HLSN) on the proliferation, apoptosis and underline molecular mechanisms of HeLa cells were investigated and compared with that of Buthus martensii (BMK). RT-PCR, Western blot and immunohistochemical staining were used to evaluate the expression levels of p21 in the control and treated cells. The present data showed that when HeLa cells were exposed to HLSN venom for 24 and 48h, the venom inhibited HeLa cell proliferation in a dose-dependent manner as determined by the methyl thiazolyl tetrazolium (MTT) assay, caused marked morphological changes as revealed by hematoxylin and eosin (HE) staining, scanning electron microscopy (SEM) and transmission electron microscopy (TEM), and induced apoptosis as shown by flow cytometry (FCM). Western blot and RT-PCR analysis revealed that the expression of p21 protein was upregulated at both transcriptional and translational levels, as supported by immunohistochemical staining. Therefore, HLSN crude venom, similar to BMK venom, inhibits proliferation and induces apoptosis in HeLa cells via the induction of p21 expression while it has little adverse effect on non cancer human umbilical vein endothelial (HUVEC-21) cells.

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