Development of a method for the quantification of fish major allergen parvalbumin in food matrix via liquid chromatography-tandem mass spectrometry with multiple reaction monitoring

文献类型： 外文期刊

第一作者： Sun, Lirui

作者： Sun, Lirui;Li, Hong;Li, Zhenxing;Ahmed, Ishfaq;Pavase, Tushar Ramesh;Sun, Weihong;Wu, Haiyan;Wang, Jianhua;Ge, Minmin

作者机构：

关键词： Allergen; Parvalbumin; Liquid chromatography; Mass spectrometry; Quantification

期刊名称：FOOD CHEMISTRY （ 影响因子：7.514； 五年影响因子：7.516 ）

ISSN： 0308-8146

年卷期： 2019 年 276 卷

页码：

收录情况： SCI

摘要： The availability of analytical methods for quantification of allergens is crucial for the correct assessment and labeling of products in order to protect allergic consumers. For this purpose, a simple, sensitive and accurate technique was developed based on liquid chromatography-tandem mass spectrometry and multiple reaction monitoring mass spectrometry (LC-MRM-MS/MS). The proposed method uses a simple purification with heat and a completely optimized tryptic digestion. This method has been validated according to the requirements defined by ICH (Q2 [R1]), having a linear range from 0.10 to 1179.36 nM with r > 0.999. The parvalbumin beta in flounder (Paralichthys olivaceus) has been quantified at a low level down to 0.10 mu g/g with satisfactory precision (RSD < 18.35%) and accuracy (< 13.3%). The new approach was successfully applied for the determination of parvalbumin beta in the other food matrices.

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