Development of an indirect ELISA assay for detecting antibodies against mammalian reovirus in pigs
文献类型： 外文期刊
第一作者： Li, Zhijie
作者： Li, Zhijie;Zhang, Xiaozhan;Hu, Xiaoliang;Tian, Jin;Kang, Hongtao;Guo, Dongchun;Liu, Jiasen;Qu, Liandong
作者机构：
关键词： Mammalian reovirus; sigma 1 protein; Indirect ELISA
期刊名称：JOURNAL OF VIROLOGICAL METHODS （ 影响因子：2.014； 五年影响因子：2.001 ）
ISSN： 0166-0934
年卷期： 2018 年 262 卷
页码：
收录情况： SCI
摘要： Mammalian reovirus (MRV) infects many species. Over the past decades, MRV infections in pigs have been reported, and several highly pathogenic MRV strains have recently been isolated in the United States. In this study, an indirect enzyme-linked immunosorbent assay (ELISA) against the sigma 1 protein from a serotype 3 reovirus strain (MPC/04) was established to detect antibodies in pigs. The assay did not react with antisera against other pig pathogens and was consistent with the indirect immunofluorescence assay (IFA) and virus neutralization test (VNT). In conclusion, the assay is specific and highly sensitive, providing a method for large-scale monitoring of the serotype 3 MRV infection epidemiology in pigs.
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