Development of an indirect ELISA assay for detecting antibodies against mammalian reovirus in pigs

文献类型： 外文期刊

第一作者： Li, Zhijie

作者： Li, Zhijie;Zhang, Xiaozhan;Hu, Xiaoliang;Tian, Jin;Kang, Hongtao;Guo, Dongchun;Liu, Jiasen;Qu, Liandong

作者机构：

关键词： Mammalian reovirus; sigma 1 protein; Indirect ELISA

期刊名称：JOURNAL OF VIROLOGICAL METHODS （ 影响因子：2.014； 五年影响因子：2.001 ）

ISSN： 0166-0934

年卷期： 2018 年 262 卷

页码：

收录情况： SCI

摘要： Mammalian reovirus (MRV) infects many species. Over the past decades, MRV infections in pigs have been reported, and several highly pathogenic MRV strains have recently been isolated in the United States. In this study, an indirect enzyme-linked immunosorbent assay (ELISA) against the sigma 1 protein from a serotype 3 reovirus strain (MPC/04) was established to detect antibodies in pigs. The assay did not react with antisera against other pig pathogens and was consistent with the indirect immunofluorescence assay (IFA) and virus neutralization test (VNT). In conclusion, the assay is specific and highly sensitive, providing a method for large-scale monitoring of the serotype 3 MRV infection epidemiology in pigs.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序