Broad-complex Z3 contributes to the ecdysone-mediated transcriptional regulation of the vitellogenin gene in Bombus lantschouensis

文献类型： 外文期刊

第一作者： Zhen, Congai

作者： Zhen, Congai;Yang, Huipeng;Luo, Shudong;Huang, Jiaxing;Wu, Jie

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期刊名称：PLOS ONE （ 影响因子：3.24； 五年影响因子：3.788 ）

ISSN： 1932-6203

年卷期： 2018 年 13 卷 11 期

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收录情况： SCI

摘要： During reproduction, vitellogenin (Vg), as an egg yolk precursor, is critical in sexually mature females of oviparous species including some insects. The transcription of Vg is usually mediated by hormones such as juvenile hormone (JH), ecdysteroids and some neuropeptides. In this study, the structure of the Vg gene from the bumblebee Bombus lantschouensis, (BIVg) was determined by sequencing and assembly. BIVg was found to be expressed at higher levels in reproductive queens than in virgins by quantitative real-time PCR analysis. Tissue-specific expression analysis showed that BIVg was expressed at the highest levels in the fat bodies of both virgin and reproductive queens. Prediction of the BIVg promoter revealed the presence of ecdysteroid-responsive cis-regulatory elements (CREs) containing one Broad-Complex zinc-finger isoform 3 (BR-C Z3), and one ecdysone-induced protein 74A (E74A). In addition, luciferase reporter expression, driven by the 5'-regulatory region of the BIVg gene, from-1517 bp to +895 bp downstream of the start codon, was induced by treatment with 20-hydroxyecdysone (20-E). Moreover, the luciferase activity of the BIVg promoter was elevated by only BIBrC-Z3 when Sf9 cells were cotransfected with four BIBrC isoforms respectively. BIVg promoter-mediated luciferase activation was significantly reduced when the putative BrC-Z3 CRE in the promoter was mutated. In summary, this report describes the first study of vitellogenin gene regulation at the transcriptional level in bumblebees and demonstrates that the ecdysone-induced transcription of the BIVg gene is mediated by the binding of BIBrC-Z3 to the BrC-Z3 CRE in the BIVg promoter in bumblebees.

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