Multiplex detection of three banana viruses by reverse transcription loop-mediated isothermal amplification (RT-LAMP)

文献类型： 外文期刊

第一作者： Zhang, Jingxin

作者： Zhang, Jingxin;Lin, Birun;Shen, Huifang;Pu, Xiaoming;Sun, Dayuan;Zhang, Jingxin;Borth, Wayne;Melzer, Michael;Nelson, Scot;Hu, John

作者机构：

关键词： Banana bunchy top virus; Banana streak OL virus; Cucumber mosaic virus; Multiplex assay; RT-LAMP

期刊名称：TROPICAL PLANT PATHOLOGY （ 影响因子：1.488； 五年影响因子：1.675 ）

ISSN： 1983-2052

年卷期： 2018 年 43 卷 6 期

页码：

收录情况： SCI

摘要： Banana bunchy top virus (BBTV), banana streak viruses (BSVs) and cucumber mosaic virus (CMV) are frequently reported infecting bananas globally. Effective control of their spread depends on robust detection of these viruses in propagation stock, planting material, infected nursery plants, and through strict quarantine. We developed single reverse transcription loop-mediated isothermal amplification (RT-LAMP) assays for BBTV, banana streak OL virus (BSV-OL) and CMV that were sensitive, specific, efficient, and completed in less than 60min. RNA-based RT-LAMP minimized false positives that arose from banana genomes harboring endogenous viral genomes, such as BSVs. RT-LAMP was also more sensitive than RT-PCR in detecting the DNA viruses, BBTV and BSV-OL, in infected plants. We also developed a multiplex assay using three sets of primers specific for each virus to simultaneously detect BBTV, BSV-OL and CMV in a sample of RNA from the same plant. The reliability and convenience of this assay makes it useful for plant quarantine and indexing plants for propagation.

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