First identification of long non-coding RNAs in fungal parasite Nosema ceranae

文献类型： 外文期刊

第一作者： Guo, Rui

作者： Guo, Rui;Chen, Dafu;Xiong, Cuiling;Zheng, Yanzhen;Fu, Zhongmin;Liang, Qin;Zhang, Lu;Wang, Hongquan;Hou, Zhixian;Hou, Chunsheng;Diao, Qingyun;Kumar, Dhiraj

作者机构：

关键词： RNA-seq; long non-coding RNA; Nosema ceranae; honey bee

期刊名称：APIDOLOGIE （ 影响因子：2.318； 五年影响因子：3.24 ）

ISSN： 0044-8435

年卷期： 2018 年 49 卷 5 期

页码：

收录情况： SCI

摘要： Nosema ceranae is a unicellular fungal parasite of honey bees and causes huge losses for apiculture. Until present, no study on N. ceranae long non-coding RNAs (lncRNAs) was documented. Here, we sequenced purified spores of N. ceranae using strand-specific library construction and high-throughput RNA sequencing technologies. In total, 83 novel lncRNAs were predicted from N. ceranae spore samples, including lncRNAs, long intergenic non-coding RNAs (lincRNAs), and sense lncRNAs. Moreover, these lncRNAs share similar characteristics with those identified in mammals and plants, such as shorter length and fewer exon number and transcript isoforms than protein-coding genes. Finally, the expression of 12 lncRNAs was confirmed with RT-PCR, confirming their true existence. To our knowledge, this is the first evidence of lncRNAs produced by a microsporidia species, offering novel insights into basic biology such as regulation of gene expression of this widespread taxonomic group.

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