Biodetoxification of Phenolic Inhibitors from Lignocellulose Pretreatment using Kurthia huakuii LAM0618(T) and Subsequent Lactic Acid Fermentation

文献类型： 外文期刊

第一作者： Xie, Yuejiao

作者： Xie, Yuejiao;Hu, Qing;Feng, Guodong;Zhao, Shumiao;Liang, Yunxiang;Peng, Nan;Jiang, Xu;Ruan, Zhiyong;Hu, Jinlong;He, Mingxiong;Hu, Guoquan;Peng, Nan

作者机构：

关键词： biodetoxification; phenolic inhibitors; Kurthia huakuii LAM0618(T); bacillus coagulans LA204; simultaneous saccharification and fermentation; lactic acid

期刊名称：MOLECULES （ 影响因子：4.411； 五年影响因子：4.587 ）

ISSN： 1420-3049

年卷期： 2018 年 23 卷 10 期

页码：

收录情况： SCI

摘要： Phenolic inhibitors generated during alkaline pretreatment of lignocellulosic biomasses significantly hinder bacterial growth and subsequent biofuel and biochemical production. Water rinsing is an efficient method for removing these compounds. Nevertheless, this method often generates a great amount of wastewater, and leads to the loss of solid fiber particles and fermentable sugars. Kurthia huakuii LAM0618(T), a recently identified microorganism, was herein shown to be able to efficiently transform phenolic compounds (syringaldehyde, hydroxybenzaldehyde, and vanillin) into less toxic acids. Taking advantage of these properties, a biodetoxification method was established by inoculating K. huakuii LAM0618(T) into the NH3/H2O2-pretreated unwashed corn stover to degrade phenolic inhibitors and weak acids generated during the pretreatment. Subsequently, 33.47 and 17.91 g/L lactic acid was produced by Bacillus coagulans LA204 at 50 degrees C through simultaneous saccharification and fermentation (SSF) from 8% (w/w) of NH3/H2O2-pretreated corn stover with or without K. huakuii LAM0618(T)-biodetoxification, indicating biodetoxification significantly increased lactic acid titer and yield. Importantly, using 15% (w/w) of the NH3/H2O2-pretreated K. huakuii LAM0618(T)-biodetoxified corn stover as a substrate through fed-batch simultaneous saccharification and fermentation, high titer and high yield of lactic acid (84.49 g/L and 0.56 g/g corn stover, respectively, with a productivity of 0.88 g/L/h) were produced by Bacillus coagulans LA204. Therefore, this study reported the first study on biodetoxification of alkaline-pretreated lignocellulosic material, and this biodetoxification method could replace water rinsing for removal of phenolic inhibitors and applied in biofuel and biochemical production using the alkaline-pretreated lignocellulosic bioresources.

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