Multi-drug resistance to antibiotics and disinfectants in Campylobacter isolates from chicken and poultry meat in Jiangsu Province, China

文献类型： 外文期刊

第一作者： Zhou, Q.

作者： Zhou, Q.;Tang, M.;Zhang, X.;Zhang, J.;Gao, Y.

作者机构：

关键词： Campylobacter; chicken; multi-drug resistant; integron; gene cassette

期刊名称：EUROPEAN POULTRY SCIENCE （ 影响因子：0.84； 五年影响因子：1.205 ）

ISSN： 1612-9199

年卷期： 2018 年 82 卷

页码：

收录情况： SCI

摘要： Objectives: To investigate the epidemiology and characterise the antimicrobial resistance profile of Campylobacter isolated from chicken and poultry meat and to explore potential multi-drug resistant (MDR) mechanisms mediated by integrons. Methods: A total of 312 Campylobacter isolates were collected from 1022 poultry meat and cloacal swab samples between 2016-2017 in Jiangsu Province, China. Antimicrobial susceptibility was tested by Kirby-Bauer (K-B) method and the minimal inhibitory concentration (MICs) of disinfectants was determined. The presence of genes encoding integrase I and integrase II were analysed by polymerase chain reaction (PCR) and molecular typing of isolates were performed by multilocus sequence typing (MLST). DNA sequencing was used to characterise gene cassettes containing the complete structure of class 1 integron. Results: Of the total collected 312 isolates, 134 strains (42.9%) contained integrase I and 7 strains contained integrase II (2.2%). The resistance to ampicillin, ceftriaxone, tobramycin, norfloxacin, ciprofloxacin, ofloxacin, enrofloxacin, sulfamethoxazole, trimethoprim, tetracycline, and lincomycin for tested isolates ranged from 56% to 100%. The MIC values of benzalkonium chloride (BC) and cetrimonium bromide (CTAB) were 0.25-64 mg/l and 0.125-1024 mg/l, respectively. A total of 55 of the isolates possessed a complete class 1 integron and most isolates contained a single gene cassette with the aminoglycoside resistance gene aadA1 located within the 1.8kb amplicon. This cassette was presented in both Campylobacter jejuni and Campylobacter coli isolates. MLST confirmed that integrase I-positive strains from poultry meat and cloacal swabs obtained from geographically distant locations shared many sequence types. Conclusions: Our data illustrates that Campylobacter resistant to both antibiotics and disinfectants are a potential risk of environmental pollution and are threatening public health which highlights the instant need for more prudent use of critically important antimicrobials. The importance of integrons for the occurrence and transmission of MDR is demonstrated.

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