Theileria annulata Cyclophilin1 (TaCyp1) Interacts With Host Cell MED21

文献类型： 外文期刊

第一作者： Zhao, Shuaiyang

作者： Zhao, Shuaiyang;Liu, Junlong;Guan, Guiquan;Liu, Aihong;Li, Youquan;Yin, Hong;Luo, Jianxun;Yin, Hong;Luo, Jianxun

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关键词： Theileria annulata; schizont; transformation; cyclophilins; MED21; NF-kappa B; interaction

期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：5.64； 五年影响因子：6.32 ）

ISSN： 1664-302X

年卷期： 2018 年 9 卷

页码：

收录情况： SCI

摘要： Host cells infected by Theileria annulata schizonts show the character of permanent proliferation in vitro, also named transformation. To explore the molecular mechanism a T. annulata Cyp1 (TaCyp1) protein potentially involved in regulating cell transformation was used as bait to screen for its interacting proteins by yeast-two-hybrid assay. Additional GST-pull down experiments confirmed that only MED21 specifically interacted with TaCyp1. Moreover, the distribution of TaCyp1 around T. annulata schizonts facilitated interaction with host cell MED21. As a component of mediator complex, MED21 is normally involved in regulating the transcription of nearly all RNA polymerase II-dependent genes. Therefore, to explore its influence on NF-kappa B signaling MED21 RNA interference and parasite killing with BW720c treatment were performed. Knock down of MED21 resulted in a significant decrease in NF-kappa B1/2 mRNA expressions, but no significant change in P105, P52 levels, nor detectable alteration in levels of phosphorylated I kappa B alpha/beta. By contrast, BW720c treatment induced an obvious decrease in the phosphorylation status of P52 and I kappa B alpha/beta, but no obvious change in that of P105. This suggests that BW720c-induced parasite death had a significant negative influence on NF-kappa B signaling, whereas knock down of MED21 had no obvious effect on NF-kappa B signaling. Characterization of TaCyp1 provides information on the function of parasite cyclophilins and leads to a better understanding of the interactions between T. annulata and its host leukocytes.

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