Transcriptome analysis reveals the molecular response to cadmium toxicity in P-pseudoannulata

文献类型： 外文期刊

第一作者： Wang, Juan

作者： Wang, Juan;Peng, Xianjin;Xu, Xiang;Wang, Zhi;Wang, Juan;Wei, Baoyang;Huang, Ting;Yang, Huilin;Sun, Zhiying;Wang, Zhi;Peng, Yuande;Xie, Chunliang;Lv, Zhiyue;Song, Qisheng

作者机构：

关键词： P. pseudoannulata; Cadmium; Detoxification; Immunity; Development

期刊名称：ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH （ 影响因子：4.223； 五年影响因子：4.306 ）

ISSN： 0944-1344

年卷期： 2018 年 25 卷 34 期

页码：

收录情况： SCI

摘要： Cadmium (Cd) can be transferred and accumulated in spiders, posing a survival risk to them. To analyze potential biological damage caused by Cd accumulation and relevant detoxification strategies employed by spiders in response to Cd exposure, we conducted transcriptome analysis of the 5th instar spider P. pseudoannulata, a common spider species playing a vital role in natural pest control in agricultural fields of southern China. We obtained 92,778 unigenes with an average length of 1104bp and identified 302, 655, and 424 differentially expressed genes (DEGs) in the spiders fed with Cd-containing fruit flies for 2, 5, and 8days, respectively. Results showed that the body mass of Cd-containing P. pseudoannulata were reduced when compared with controls, presumably due to delayed maturation of tissues and organs. Meanwhile, functional analysis of DEGs indicated that Cd may have a negative effect on neural signal transduction and molt cycle of the spider. For defense strategies, detoxification enzymes like glutathione S-transferase (GST), catalase (CAT), superoxide dismutase (SOD), and P450, and typical proteins like heat shock protein and metallothionein were all differentially expressed in response to Cd stress. Besides, innate immune responses like toll-like receptor signaling pathways were also upregulated. Multiple critical Cd-responsive genes involved in biological damage, detoxification, and immune response were identified, providing referable foundation for further research on Cd toxicity to P. pseudoannulata.

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