文献类型： 外文期刊
第一作者： Yang, Shupeng
作者： Yang, Shupeng;Yi, Xiujuan;Mao, Xin;Li, Yanshen;Liu, Yunguo;Zhang, Suxia
作者机构：
关键词： PI-PO; multi-classes analysis; integrated immunoassay; biosensor
期刊名称：FOOD AND AGRICULTURAL IMMUNOLOGY （ 影响因子：3.101； 五年影响因子：2.573 ）
ISSN： 0954-0105
年卷期： 2018 年 29 卷 1 期
页码：
收录情况： SCI
摘要： Europium (Eu3+) and horse radish peroxidase (HRP) are highly promising biosensors due to its excellent physical and chemical properties. The two biosensors are widely applied in time-resolved fluoroimmunoassay (TRFIA) and chemiluminescence enzymelinked immunosorbent assay, respectively. In this work, the two technologies were integrated with parallel input and parallel output (PI-PO) characters. Eu3+ was adopted as probe I in TRFIA for deoxynivalenol and de-epoxyldexoylnivalenol with a limit of detection (LOD) of 4.74 and 4.56 ng/mL, while HRP was performed as probe II in CL-ELISA for FB1 with an LOD of 0.088 ng/mL. Compared to the traditional individual assay, this method exhibited fast and broad detection characteristics by integrating two immunoassays into one.
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