Computational modeling and functional characterization of a GgChi: A class III chitinase from corms of Gladiolus grandiflorus

文献类型： 外文期刊

第一作者： Shah, Kausar Hussain

作者： Shah, Kausar Hussain;Khaliq, Binish;Baig, Mirza Ahsen;Mahmood, Seema;Akrem, Ahmed;Hussain, Ashiq;Saeed, Qamar;Sial, Muhammad Umair;Ali, Zahid;Buck, Friedrich;Goodman, Richard E.;Ishaq, Uzma;Munawar, Aisha

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关键词： Gladiolus grandiflorus; Endochitinase; TIM barrel; Antibacterial; Antifungal

期刊名称：KAOHSIUNG JOURNAL OF MEDICAL SCIENCES （ 影响因子：2.744； 五年影响因子：2.296 ）

ISSN： 1607-551X

年卷期： 2018 年 34 卷 12 期

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收录情况： SCI

摘要： The present study describes the predicted model and functional characterization of an endochitinase (30 kDa) from corms of Gladiolus grandiflorus. ESI-QTOF-MS generated peptide showed 96% sequence homology with family 18, Class III acidic endochitinase of Gladiolus gandavensis. Purified G. grandiflorus chitinase (GgChi) hydrolyzed 4-methylumbelliferyl beta-D-N,N',N ''-triacetylchitotriose substrate showing specific endochitinase activity. Since no structural details of GgChi were available in the Protein Data Bank (PDB), a homology model was predicted using the coordinate information of Crocus vernus chitinase (PDB ID: 3SIM). Ramachandran plot indicated 84.5% in most favored region, 14.8% in additional and 0.6% in generously allowed region while no residue in disallowed region. The predicted structure indicated a highly conserved (beta/alpha)(8) (TIM barrel) structure similar to the family 18, class III chitinases. The GgChi also showed sequence and structural homologies with other active chitinases. The GgChi (50 mu g/disc) showed no antibacterial activity, but did provide mild growth inhibition of phytopathogenic fungus Fusarium oxysporum at a concentration of 500 mu g/well Similarly, insect toxicity bioassays of GgChi (50 mu g) against nymphs of Bemisia tabaci showed 14% reduction in adult emergence and 14% increase in mortality rate in comparison to control values. The GgChi (1.5 mg) protein showed significant reduction in a population of flour beetle (Tribolium castaneum) after 35 days, but lower reactivity against rice weevil (Sitophilus oryzae). The results of this study provide detai.led insight on functional characterization of a family 18 class III acidic plant endochitinase. Copyright (C) 2018, Kaohsiung Medical University. Published by Elsevier Taiwan LLC.

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