Characterization of a nucleus located mollusc mitoferrin and its response to OsHV-1 infection

文献类型： 外文期刊

第一作者： Xin, Lusheng

作者： Xin, Lusheng;Li, Chen;Bai, Changming;Wang, Chongming;Xin, Lusheng;Li, Chen;Bai, Changming;Wang, Chongming;Huang, Bowen

作者机构：

关键词： mitoferrin; mitochondria; iron metabolism; Scapharca broughtonii; OsHV-1 infection

期刊名称：BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS （ 影响因子：3.77； 五年影响因子：4.137 ）

ISSN： 0304-4165

年卷期： 2019 年 1863 卷 1 期

页码：

收录情况： SCI

摘要： Mitoferrin genes as members of SLC25 family are conservatively existed across species, mainly locate on mitochondria and serve an important role in the regulation of whole cellular iron metabolism. Available iron withholding from pathogens presents an important host defense strategy, while the regulation role of mitoferrin against invading pathogens is largely unknown. In this study, a unique mollusc mitoferrin gene was identified in ark clams, named SbmiFn, that showed conserved three-dimensional structure with other mitoferrins, and its iron binding activity was verified by iron chelating assay. Besides cytoplasmic distribution, colocalization between SbmiFn and nuclei was observed by immunohistochemistry assay. Moreover, the response of SbmiFn to viral pathogen OsHV-1 was investigated. The results showed that nucleus located signal of SbmiFn was enhanced, the expressions of SbmiFn and ferritin were coordinately decreased, which might assist host against OsHV-1 replication as the increase of OsHV-1 copies were hardly detected after that. These results refreshed our knowledge on the sequence, structure and functional characteristics of mitoferrin subfamily, and would contribute to further comparative studies on iron metabolism.

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