Response of detoxification and immune genes and of transcriptome expression in Mythimna separata following chlorantraniliprole exposure

文献类型： 外文期刊

第一作者： Wang, Jin-Da

作者： Wang, Jin-Da;Wang, Wei-Zhong;Wang, Ya-Ru;Gao, San-Ji;Elzaki, Mohammed;Wu, Min;Wang, Ran;Wang, Rong;Wu, Min

作者机构：

关键词： Mythimna separata; Transcriptome; Ryanodine receptor; P450 genes; Chlorantraniliprole

期刊名称：COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS （ 影响因子：2.674； 五年影响因子：2.941 ）

ISSN： 1744-117X

年卷期： 2018 年 28 卷

页码：

收录情况： SCI

摘要： The oriental armyworm Mythimna separata is a serious polyphagous pest in China and there are major efforts to control this pest. In the present study, an RNA-Seq method was used to explore transcriptome data of M. separata and identify the responses of genes to chlorantraniliprole. Sequencing and de novo assembly yielded 134,533 transcripts that were further assembled into 77,628 unigenes with an N50 length of 2165 bp. A total of 76 unigenes encoding insecticide targets were identified. Furthermore, 62 cytochrome P450s, 34 glutathione Stransferase (GSTs)and 64 carboxylesterase (CCEs) were curated to construct phylogenetic trees. In addition, we identified 647 the differentially expressed genes following treatment with chlorantraniliprole. The pathways of calcium signaling was identified as response to the pesticide The transcriptome data we generated represents a comprehensive genomic resource for further studies focused on control of M. separata. The response of genes to chlorantraniliprole treatment will elucidate the molecular mechanisms of insecticide resistance and allow for the development of new chemical pesticides to control this pest.

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