The autosomal Gsdf gene plays a role in male gonad development in Chinese tongue sole (Cynoglossus semilaevis)

文献类型： 外文期刊

第一作者： Zhu, Ying

作者： Zhu, Ying;Meng, Liang;Xu, Wenteng;Cui, Zhongkai;Zhang, Nianwei;Guo, Hua;Wang, Na;Shao, Changwei;Chen, Songlin;Zhu, Ying;Meng, Liang;Xu, Wenteng;Cui, Zhongkai;Zhang, Nianwei;Guo, Hua;Wang, Na;Shao, Changwei;Chen, Songlin

作者机构：

期刊名称：SCIENTIFIC REPORTS （ 影响因子：4.379； 五年影响因子：5.133 ）

ISSN： 2045-2322

年卷期： 2018 年 8 卷

页码：

收录情况： SCI

摘要： Gsdf is a key gene for testicular differentiation in teleost. However, little is known about the function of Gsdf in Chinese tongue sole (Cynoglossus semilaevis). In this study, we obtained the full-length Gsdf gene (CS-Gsdf), and functional characterization revealed its potential participation during germ cell differentiation in testes. CS-Gsdf transcription was predominantly detected in gonads, while the levels in testes were significantly higher than those in ovaries. During the different developmental stages in male gonads, the mRNA level was significantly upregulated at 86 dph, and a peak appeared at 120 dph; then, the level decreased at 1 and 2 yph. In situ hybridization revealed that CS-Gsdf mRNA was mainly localized in the Sertoli cells, spermatogonia, and spermatids in mature testes. After CS-Gsdf knockdown in the male testes cell line by RNA interference, a series of sex-related genes was influenced, including several sex differentiation genes, CS-Wnt4 alpha, CS-Cyp19 alpha 1 alpha and CS-Star. Based on these data, we speculated that CS-Gsdf may play a positive role in germ differentiation and proliferation via influencing genes related to sex differentiation.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序