Recombinant infectious laryngotracheitis virus expressing Newcastle disease virus F protein protects chickens against infectious laryngotracheitis virus and Newcastle disease virus challenge

文献类型： 外文期刊

第一作者： Shao, Yuhao

作者： Shao, Yuhao;Sun, Junfeng;Han, Zongxi;Liu, Shengwang

作者机构：

关键词： Infectious laryngotracheitis virus; Newcastle disease virus; Genotype VII; Bivalent vaccine candidate

期刊名称：VACCINE （ 影响因子：3.641； 五年影响因子：3.816 ）

ISSN： 0264-410X

年卷期： 2018 年 36 卷 52 期

页码：

收录情况： SCI

摘要： In this study, we isolated and identified an infectious laryngotracheitis virus (ILTV) that was naturally avirulent in specific pathogen-free (SPF) chickens, with the aim of developing a more efficacious vaccine against ILTV and Newcastle disease virus (NDV). We constructed a US9-deleted ILTV mutant based on this avirulent ILTV, and then constructed a recombinant ILTV (designated ILTV-Delta US9-F) expressing the fusion protein (F) of the genotype VII NDV based on the US9-deleted ILTV mutant. Expression of the F protein in ILTV-Delta US9-F-infected cells was confirmed by indirect immunofluorescence assay and western blotting. The inserted F gene was stably expressed in ILTV-Delta US9-F. The growth kinetics of ILTV-Delta US9-F were comparable to those of the wild-type ILTV strain. Vaccination of SPF chickens with ILTV-Delta US9-F produced no clinical signs but did induce low levels of NDV-specific enzyme-linked immunosorbent assay and neutralizing antibodies. A single vaccination with 10(4) plaque-forming units (PFU) of ILTV-Delta US9-F provided good protection against both genotype VII and IX NDVs based on clinical signs, similar to the protection provided by the commercial live La Sota vaccine. Notably, ILTV-Delta US9-F limited the replication and shedding of genotype VII NDV from oropharyngeal swabs more efficiently than the La Sota vaccine. In addition, vaccination with lower doses (10(3) and 10(2) PFU) of ILTV-Delta US9-F also provided sufficient clinical protection. These results indicated that ILTV-Delta US9-F may be a bivalent vaccine candidate against both ILTV and NDV. (C) 2018 Elsevier Ltd. All rights reserved.

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