Development of a live attenuated vaccine against Muscovy duck reovirus infection

文献类型： 外文期刊

第一作者： Chen, Shilong

作者： Chen, Shilong;Lin, Fengqiang;Chen, Shaoying;Hu, Qilin;Cheng, Xiaoxia;Jiang, Bin;Zhu, Xiaoli;Wang, Shao;Zheng, Min;Huang, Meiqing

作者机构：

关键词： Muscovy duck reovirus (MDRV); Live attenuated vaccine; Safety; Stability; Protective efficacy

期刊名称：VACCINE （ 影响因子：3.641； 五年影响因子：3.816 ）

ISSN： 0264-410X

年卷期： 2018 年 36 卷 52 期

页码：

收录情况： SCI

摘要： The Muscovy duck reovirus (MDRV) is a highly pathogenic virus that causes substantial economic losses in the Muscovy duck industry. While MDRV poses a significant threat to Muscovy ducklings, no vaccine candidates are available to date to alleviate MDRV infection throughout the world. The present study presents efforts toward establishing an attenuated vaccine for MDRV. For this purpose, a live attenuated vaccine strain named CA was obtained via alternate propagation of the MDRV isolate MW9710 in both Muscovy duck embryo fibroblasts (MDEFs) and chicken embryo fibroblasts (CEFs) for 90 passages. The CA strain achieved an adaptive growth capacity in CEFs with a viral titer that ranged between 10(5.5) TCID50/100 mu L and lost its pathogenicity in 1-day-old Muscovy ducklings. Compared to the parent strain MW9710, the CA strain has 42 scattered amino acid substitutions, most of which are located in the lambda B, lambda C, mu B,sigma B and sigma C protein. The CA strain maintained its attenuation and showed no gene mutation or virulence reversion after back propagation into 1-day-old ducklings for five rounds. The minimum protective dose was calculated to be 300 TCID50 of the CA strain. Furthermore, a single dose of CA vaccine protected immunized ducklings against lethal challenge by the virulent MDRV strain MW9710 and significantly decreased viral loads. In summary, the CA strain exhibited striking genetic stability, excellent safety, and effective immunogenicity. This CA strain of MDRV is a promising vaccine candidate for the prevention and control of MDRV infection. (C) 2018 Elsevier Ltd. All rights reserved.

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