Fungal resistance mediated by maize wall-associated kinase ZmWAK-RLK1 correlates with reduced benzoxazinoid content

文献类型： 外文期刊

第一作者： Yang, Ping

作者： Yang, Ping;Praz, Coraline;Singla, Jyoti;Luethi, Linda;Krattinger, Simon G.;Keller, Beat;Yang, Ping;Li, Beibei;Robert, Christelle A. M.;Erb, Matthias;Kessel, Bettina;Scheuermann, Daniela;Ouzunova, Milena;Krattinger, Simon G.

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关键词： benzoxazinoids (BXs); Htn1; maize disease resistance; receptor-like kinase; wall-associated kinase (WAK)

期刊名称：NEW PHYTOLOGIST （ 影响因子：10.151； 五年影响因子：10.475 ）

ISSN： 0028-646X

年卷期： 2019 年 221 卷 2 期

页码：

收录情况： SCI

摘要： Wall-associated kinases (WAKs) have recently been identified as major components of fungal and bacterial disease resistance in several cereal crop species. However, the molecular mechanisms of WAK-mediated resistance remain largely unknown. Here, we investigated the function of the maize gene ZmWAK-RLK1 (Htn1) that confers quantitative resistance to northern corn leaf blight (NCLB) caused by the hemibiotrophic fungal pathogen Exserohilum turcicum. ZmWAK-RLK1 was found to localize to the plasma membrane and its presence resulted in a modification of the infection process by reducing pathogen penetration into host tissues. A large-scale transcriptome analysis of near-isogenic lines (NILs) differing for ZmWAK-RLK1 revealed that several differentially expressed genes are involved in the biosynthesis of the secondary metabolites benzoxazinoids (BXs). The contents of several BXs including DIM(2)BOA-Glc were significantly lower when ZmWAK-RLK1 is present. DIM(2)BOA-Glc concentration was significantly elevated in ZmWAK-RLK1 mutants with compromised NCLB resistance. Maize mutants that were affected in overall BXs biosynthesis or content of DIM(2)BOA-Glc showed increased NCLB resistance. We conclude that Htn1-mediated NCLB resistance is associated with a reduction of BX secondary metabolites. These findings suggest a link between WAK-mediated quantitative disease resistance and changes in biochemical fluxes starting with indole-3-glycerol phosphate.

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