Structural Insights into the Preferential Binding of PGRP-SAs from Bumblebees and Honeybees to Dap-Type Peptidoglycans Rather than Lys-Type Peptidoglycans

文献类型： 外文期刊

第一作者： Liu, Yanjie

作者： Liu, Yanjie;Zhao, Xiaomeng;Huang, Jiaxing;Chen, Minming;An, Jiandong

作者机构：

期刊名称：JOURNAL OF IMMUNOLOGY （ 影响因子：5.422； 五年影响因子：6.029 ）

ISSN： 0022-1767

年卷期： 2019 年 202 卷 1 期

页码：

收录情况： SCI

摘要： The peptidoglycan recognition protein SAs (PGRP-SAs) from Bombus ignitus (Bi-PGRP-SA), Apis mellifera (Am-PGRP-SA), and Megachile rotundata PGRP-SA (Mr-PGRP-SA) exhibit an intrinsic ability to preferentially bind to Dap-type peptidoglycan (PGN) from Bacillus subtilis rather than Lys-type PGN from Micrococcus luteus. This ability is more analogous to the binding exhibited by PGRP-LCx and PGRP-SD than to that exhibited by PGRP-SA in Drosophila. Moreover, Bi-PGRP-SA and Am-PGRP-SA share greater sequence identity with Drosophila PGRP-LCx than with PGRP-SD and retain several conserved contact residues, including His(37)/His(38), His(60)/His(61), Trp(66)/Trp(67), Ala(150)/Ala(151), and Thr(151)/Thr(152). However, the corresponding contact residue Arg(85) is not a major anchor residue in bees (e.g., bumblebees, honeybees, and leaf-cutting bees), and an in silico analysis indicated that the residues Thr(151)/Thr(152) and Ser(153)/Ser(154) of Bi-PGRP-SA and Am-PGRP-SA are deduced to be anchor residues. In addition, the nonconserved residues Asp(67) in Bi-PGRP-SA and Mr-PGRP-SA and His(68) in Am-PGRP-SA are deduced to be involved in the binding to Dap-type PGNs in bumblebees, honeybees, and leaf-cutting bees. We conclude that the structures and specificities of PGRP-SAs in bees are more analogous to those of PGRP-LCx than to those of Drosophila PGRP-SA. This phenomenon might be explained by the fact that the evolutionary clade of Hymenoptera is more ancient than that of Diptera.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序