Effect of shRNA-mediated Xist knockdown on the quality of porcine parthenogenetic embryos

文献类型： 外文期刊

第一作者： Chen, Xiaoyu

作者： Chen, Xiaoyu;Zhu, Zhiwei;Yu, Fuxian;Huang, Jing;Jia, Ruoxin;Pan, Jianzhi

作者机构：

关键词： blastocyst cell number; RNA interference; parthenogenesis; pig; pluripotent transcription factors

期刊名称：DEVELOPMENTAL DYNAMICS （ 影响因子：3.78； 五年影响因子：3.476 ）

ISSN： 1058-8388

年卷期： 2019 年 248 卷 1 期

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收录情况： SCI

摘要： Background: Parthenogenetically activated oocytes exhibit poor embryo development and lower total numbers of cells per blastocyst accompanied by abnormally increased expression of Xist, a long noncoding RNA that plays an important role in triggering X chromosome inactivation during embryogenesis. Results: To investigate whether knockdown of Xist influences parthenogenetic development in pigs. We developed an anti-Xist short hairpin RNA (shRNA) vector, which can significantly inhibit Xist expression for at least seven days when injected at 12-13 hr after parthenogenetic activation. Embryonic cleavage, blastocyst formation, and total blastocyst cell numbers were compared during the blastocyst stage, as well as the expression of an X-linked gene and three pluripotent transcription factors. Knockdown of Xist significantly increases the total blastocyst cell number, but does not influence the rate of embryo cleavage and blastocyst formation. The expressions of Sox2, Nanog, and Oct4 were also significantly improved in the injected embryos compared with the control at the blastocyst stage, but the Foxp3 expression level was not increased significantly. Conclusions: The present study provides valuable information for understanding the role of Xist in parthenogenesis and presents a new approach for improving the quality of porcine parthenogenetic embryos. Developmental Dynamics 248:140-148, 2019. (c) 2018 Wiley Periodicals, Inc.

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