Development and application of a colloidal gold test strip for detection of avian leukosis virus

文献类型： 外文期刊

第一作者： Yu, Mengmeng

作者： Yu, Mengmeng;Bao, Yuanling;Xing, Lixiao;Chang, Fangfang;Liu, Yongzhen;Farooque, Muhammad;Wang, Yongqiang;Qi, Xiaole;Liu, Changjun;Zhang, Yanping;Cui, Hongyu;Li, Kai;Gao, Li;Pan, Qing;Wang, Xiaomei;Gao, Yulong;Wang, Muping;Zhu, Haibo;Wang, Xiaoyan

作者机构：

关键词： Avian leukosis virus; Colloidal gold strip test; Eradication; ELISA; Meconium

期刊名称：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY （ 影响因子：4.813； 五年影响因子：4.697 ）

ISSN： 0175-7598

年卷期： 2019 年 103 卷 1 期

页码：

收录情况： SCI

摘要： Avian leukosis virus (ALV) is an avian oncogenic retrovirus that induces leukemia-like proliferative diseases in chickens. ALV infection can result in the development of immunological tolerance and persistent viremia. Since effective vaccines against ALV are not yet available, its current prevention primarily depends on detection and eradication to establish exogenous ALV-free poultry flocks. In this study, a rapid and simple colloidal gold test strip method, specific for the group-specific antigen, p27 protein, was developed and systematically evaluated for the detection of ALV from different samples. The detection limit of this assay was as low as 6.25ng/ml for p27 protein and 80 TCID50/ml for different subgroups of ALV. Besides, the test strip showed high specificity in the detection of different subgroups of ALV, including ALV-A, ALV-B, ALV-J, and ALV-K, with no cross-reaction with other avian pathogens. Furthermore, we artificially infected specific pathogen-free (SPF) chickens with ALV-J, collected cloacal swabs, and examined viral shedding using both test strips and ELISA. Results from the test strip were highly consistent with that from ELISA. In addition, 1104 virus isolates from anti-coagulant blood samples, 645 albumen samples, and 4312 meconium samples were tested, and the test strip results agreed with those of ELISA kit up to 97.1%. All the results indicated that the colloidal gold test strip could serve as a simple, rapid, sensitive, and specific diagnostic method for eradication of ALV in poultry farms.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序