Hi-TOM: a platform for high-throughput tracking of mutations induced by CRISPR/Cas systems

文献类型： 外文期刊

第一作者： Liu, Qing

作者： Liu, Qing;Wang, Chun;Jiao, Xiaozhen;Wang, Kejian;Zhang, Huawei;Gao, Caixia;Song, Lili;Li, Yanxin

作者机构：

关键词： CRISPR; Cas; genome editing; mutation identification; Hi-TOM

期刊名称：SCIENCE CHINA-LIFE SCIENCES （ 影响因子：6.038； 五年影响因子：4.754 ）

ISSN： 1674-7305

年卷期： 2019 年 62 卷 1 期

页码：

收录情况： SCI

摘要： The CRISPR/Cas system has been extensively applied to make precise genetic modifications in various organisms. Despite its importance and widespread use, large-scale mutation screening remains time-consuming, labour-intensive and costly. Here, we developed Hi-TOM (available at https://doi.org/www.hi-tom.net/hi-tom/), an online tool to track the mutations with precise percentage for multiple samples and multiple target sites. We also described a corresponding next-generation sequencing (NGS) library construction strategy by fixing the bridge sequences and barcoding primers. Analysis of the samples from rice, hexaploid wheat and human cells reveals that the Hi-TOM tool has high reliability and sensitivity in tracking various mutations, especially complex chimeric mutations frequently induced by genome editing. Hi-TOM does not require special design of barcode primers, cumbersome parameter configuration or additional data analysis. Thus, the streamlined NGS library construction and comprehensive result output make Hi-TOM particularly suitable for high-throughput identification of all types of mutations induced by CRISPR/Cas systems.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序