NRDR inhibits estradiol synthesis and is associated with changes in reproductive traits in pigs

文献类型： 外文期刊

第一作者： Liu, Ying

作者： Liu, Ying;Li, Wentong;Zhang, Yanmin;Ao, Hong;Zhou, Rong;Li, Kui;Yang, Yalan;Yang, Yanzhao;Li, Hua;Li, Wentong;Geng, Zhaoyu

作者机构：

关键词： cumulus cells; NADPH-dependent retinol dehydrogenase/reductase (NRDR); pig; reproduction traits; steroid hormone biosynthesis

期刊名称：MOLECULAR REPRODUCTION AND DEVELOPMENT （ 影响因子：2.609； 五年影响因子：3.47 ）

ISSN： 1040-452X

年卷期： 2019 年 86 卷 1 期

页码：

收录情况： SCI

摘要： Cumulus cells secreting steroid hormones have important functions in oocyte development. Several members of the short-chain dehydrogenase/reductase (SDR) family are critical to the biosynthesis of steroid hormones. NADPH-dependent retinol dehydrogenase/reductase (NRDR), a member of the SDR superfamily, is overexpressed in pig breeds that also show high levels of androstenone. However, the potential functions and regulatory mechanisms of NRDR in pig ovaries have not been reported to date. The present study demonstrated that NRDR is highly expressed in pig ovaries and is specifically located in cumulus granulosa cells. Functional studies showed that NRDR inhibition increased estradiol synthesis. Both pregnant mare serum gonadotropin and human chorionic gonadotropin downregulated the expression of NRDR in pig cumulus granulosa cells. When the relationship between reproductive traits and single-nucleotide polymorphisms (SNPs) of the NRDR gene was examined, we found that two SNPs affected reproductive traits. SNP rs701332503 was significantly associated with a decrease in the total number of piglets born during multiparity, and rs326982309 was significantly associated with an increase in the average birth weight during primiparity. Thus, NRDR has an important role in steroid hormone biosynthesis in cumulus granulosa cells, and NRDR SNPs are associated with changes in porcine reproduction traits.

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