Structural characteristics and succession of intestinal microbiota for Paralichthys olivaceus during the early life stage

文献类型： 外文期刊

第一作者： Jiang, Yan

作者： Jiang, Yan;Liu, Zengxin;Liu, Xuezhou;Xu, Yongjiang;Shi, Bao;Wang, Bin;Jiang, Yan;Liu, Xuezhou;Xu, Yongjiang;Shi, Bao;Wang, Bin

作者机构：

关键词： influential factor; intestinal microbiota; larva and juvenile; Paralichthys olivaceus; structural characteristic

期刊名称：AQUACULTURE RESEARCH （ 影响因子：2.082； 五年影响因子：2.415 ）

ISSN： 1355-557X

年卷期： 2019 年 50 卷 2 期

页码：

收录情况： SCI

摘要： The research of intestinal microflora structures for Paralichthys olivaceus larvae and juveniles will help us to master the ontogeny and developmental colonization of microflora during the larval rearing stage. In this study, we sequenced the total bacterial genomic DNA in larval and juvenile guts with an Illumina MiSeq PE300 system, and analysed the structural characteristics of these microbiota, feed and rearing water in live and formulated feeding periods. The structure of gut microbiota was gradually similar to those in wild P. olivaceus at the phylum level and the newly hatched ones with the growth, according to the distribution and abundance of intestinal microbiota. And the colonized rule of main microbial species in guts was decreased initially and then increased during the larvae and juveniles stage. Meantime, the core microflora of this study were obtained through the analysis of shared and dominant species, which included Bacteroides, Bacillus, Enterococcus, Lactobacillus, Lactococcus, Escherichia_Shigella, Acinetobacter, Pseudomonas, Vibrio, Nitrosomonas, and Glaciecola. The correlation analysis of microbiota between intestines and environmental factors suggested that microflora in feed and water could affect the distribution of larval and juvenile gut microbiota. Moreover, many species of Acinetobacter, Pseudomonas and Vibrio are treated as important potential pathogens in aquaculture. These all pointed out the microbial quality of feed and rearing water should be strictly controlled in fish breeding and farming, and supply theoretical bases for screening the native probiotics to artificially regulate the gut microbiota.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序