PHYSIOLOGICAL, PHYLOGENETIC AND FUNCTIONAL IDENTIFICATION OF CRUDE OIL-DEGRADING MICROBIAL POPULATIONS

文献类型： 外文期刊

第一作者： Liu, Yujia

作者： Liu, Yujia;Zhao, Yuan;Zhu, Zhibao;Li, Muwang;Liang, Yuting

作者机构：

关键词： biodegradation; functional gene analysis; molecular identification; oil pollution

期刊名称：ENVIRONMENTAL ENGINEERING AND MANAGEMENT JOURNAL （ 影响因子：0.916； 五年影响因子：0.869 ）

ISSN： 1582-9596

年卷期： 2018 年 17 卷 11 期

页码：

收录情况： SCI

摘要： In this study, nine microbial strains with oil-degrading function were isolated from oil-contaminated soil near four oil wells in the 0.6 km(2) area of the Jinnan Oil Field, Huaian, Jiangsu, China (32 degrees 57' N, 119 degrees 02' E). Based on morphological observations, physiological and biochemical responses, and molecular identification, we confirmed that the nine strains belong to five genera: Pseudomonas, Raoultella, Lysinibacillus, Escherichia, and Klebsiella. The degradation capacity of the nine strains in crude oil medium ranged from 2.43% to 32.63% with an average rate of 21.64%. Further plasmid and chromosome analyses on the oil-degrading genes of the nine microbial strains were conducted. The GST gene (degradable polycyclic aromatics) was widely distributed in the chromosomes of eight strains and the plasmids of three strains. The alkB gene was detected in the chromosomes of seven strains and the plasmids of two strains. The LmPH gene (degradable phenolic substances) was only detected in the chromosome of one strain. Molecular biological analyses on the isolation, identification, and degradation characteristics of oil-degrading microbial strains provide a foundation for microbial remediation in oil-polluted environments.

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