The WRKY Transcription Factor GmWRKY12 Confers Drought and Salt Tolerance in Soybean

文献类型： 外文期刊

第一作者： Shi, Wen-Yan

作者： Shi, Wen-Yan;Min, Dong-Hong;Zhang, Xiao-Hong;Shi, Wen-Yan;Du, Yong-Tao;Jin, Long-Guo;Chen, Jun;Chen, Ming;Zhou, Yong-Bin;Ma, You-Zhi;Xu, Zhao-Shi;Ma, Jian

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关键词： WRKY; stress responsive mechanism; drought tolerance; salt tolerance; transgenic hairy root assay; soybean

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.923； 五年影响因子：6.132 ）

ISSN： 1422-0067

年卷期： 2018 年 19 卷 12 期

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收录情况： SCI

摘要： WRKYs are important regulators in plant development and stress responses. However, knowledge of this superfamily in soybean is limited. In this study, we characterized the drought- and salt-induced gene GmWRKY12 based on RNA-Seq and qRT-PCR. GmWRKY12, which is 714 bp in length, encoded 237 amino acids and grouped into WRKY II. The promoter region of GmWRKY12 included ABER4, MYB, MYC, GT-1, W-box and DPBF cis-elements, which possibly participate in abscisic acid (ABA), drought and salt stress responses. GmWRKY12 was minimally expressed in different tissues under normal conditions but highly expressed under drought and salt treatments. As a nucleus protein, GmWRKY12 was responsive to drought, salt, ABA and salicylic acid (SA) stresses. Using a transgenic hairy root assay, we further characterized the roles of GmWRKY12 in abiotic stress tolerance. Compared with control (Williams 82), overexpression of GmWRKY12 enhanced drought and salt tolerance, increased proline (Pro) content and decreased malondialdehyde (MDA) content under drought and salt treatment in transgenic soybean seedlings. These results may provide a basis to understand the functions of GmWRKY12 in abiotic stress responses in soybean.

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