Effect of Heat Treatment on the Antitumor Activity of Lactoferrin in Human Colon Tumor (HT29) Model

文献类型： 外文期刊

第一作者： Li, Hui-Ying

作者： Li, Hui-Ying;Yang, Huai-Gu;Li, Peng;Wang, Yi-Zhen;Huang, Guo-Xin;Xing, Lei;Wang, Jia-Qi;Zheng, Nan

作者机构：

关键词： lactoferrin; antitumor activity; HT29 cell; heat treatment; tumor-bearing model

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：5.279； 五年影响因子：5.269 ）

ISSN： 0021-8561

年卷期： 2019 年 67 卷 1 期

页码：

收录情况： SCI

摘要： To investigate the effect of heat treatment on the antitumor activity of lactoferrin in colon cancer cells and colon tumors, the HT-29 (human intestinal epithelial tumor cell) cell line was exposed to lactoferrin and various heat treatments. The impacts on cell proliferation, invasion, and migration were observed in vitro, and nude mice bearing HT29 tumors were administered lactoferrin and underwent various heat treatments in vivo. In the HT29 cell proliferation test using transwell and scratch analyses, lactoferrin (20 mg/mL) without or with heat treatment (50 and 70 degrees C) significantly inhibited cell proliferation, migration, and invasion (compared with the control, p < 0.05), while lactoferrin with heat treatment (100 degrees C) did not affect these parameters. In vivo, HT29 tumor weight was significantly reduced in the lactoferrin (without heat treatment and with 50 and 70 degrees C treatment) groups (1.59 +/- 0.20, 1.67 +/- 0.25, and 2.41 +/- 0.42 g, compared with the control, p < 0.05), and there was no significant difference between the control (3.73 +/- 0.33 g) and the 100 degrees C treatment group (3.58 +/- 0.29 g). Moreover, 100 degrees C heat treatment reduced inhibition of the VEGFR2/VEGFA/PI3K/Akt/Erkl/2 angiogenesis pathway by lactoferrin. In summary, HT29 tumors were effectively suppressed by lactoferrin via inhibition of VEGFR2/VEGFA/PI3K/Akt/Erkl/2 pathway, and heat treatment affected the antitumor activity of lactoferrin in a temperature-dependent manner.

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