Isolation, cloning and characterization of an azoreductase and the effect of salinity on its expression in a halophilic bacterium

文献类型： 外文期刊

第一作者： Tian, Fang

作者： Tian, Fang;Guo, Guang;Yang, Feng;Hu, Zhixin;Guo, Guang;Hu, Zhixin;Zhang, Can;Liu, Chong;Wang, Shi-wei

作者机构：

关键词： Azo dye; Halophilic bacteria; Azoreductase

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：6.953； 五年影响因子：6.737 ）

ISSN： 0141-8130

年卷期： 2019 年 123 卷

页码：

收录情况： SCI

摘要： Understanding the molecular mechanisms of azo dye decolorization is important for the development of effective bioremediation for textile-colored wastewater. A halophilic bacterium Halomonas sp. strain GT was isolated, which could degrade the azo dye Acid Brilliant Scarlet GR at 10% NaCl. The complete genome sequence of this strain was obtained using the PacBio RS II platform. Genome annotation revealed that four proteins are related to decolorization of azo dyes, such as azoreductase, laccases, benzene 1,2-dioxygenase, and catechol 1,2-dioxygenase. The putative azoreductase gene of Halomonas sp. strain GT responsible for the decolorization of azo dye in high salt environment was isolated. Phylogenetic tree analysis showed that the azoG (azoreductase gene of Halomonas sp. strain GT) and its homologs constituted a new branch of the NADH depending azoreductases, with all the homologous sequence of the protein from halophilic bacteria. At high NaCl concentrations, azoreductase gene expression and azoreductase activity were restrained in Halomonas sp. strain GT, which resulted in low a decolorization rate. (C) 2018 Published by Elsevier B.V.

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