Comparative analysis of nitrogen spatial heterogeneity responses in low nitrogen susceptible and tolerant tea plants (Camellia sinensis)

文献类型： 外文期刊

第一作者： Ruan, Li

作者： Ruan, Li;Wang, Liyuan;Wei, Kang;Cheng, Hao;Li, Hailin;Shao, Shujun;Wu, Liyun;Ruan, Li

作者机构：

关键词： N spatial heterogeneity; Root growth; Free amino acid; Gene expression; N transportation; N assimilation

期刊名称：SCIENTIA HORTICULTURAE （ 影响因子：3.463； 五年影响因子：3.672 ）

ISSN： 0304-4238

年卷期： 2019 年 246 卷

页码：

收录情况： SCI

摘要： As a leaf crop, tea plants consume a high amount of nitrogen (N), but the physiological and molecular mechanisms of tea in response to N spatial heterogeneity are poorly understood. Based on a common planting pattern of unilaterally fertilized double rows, the phenotype, free amino acid (FAA) contents, N contents of the soil and plants, root growth and gene expression of contrasting tea genotypes (Longjing43 (LJ43) and Liyou002 (LY002)) were compared in this study. Our results showed that, the unilaterally fertilized double row cultivation system led to N spatial heterogeneity in soil. For field growth, the ratio of young to mature leaves, leaf SPAD value, and differences between the two lines (near (-near) and far from (-far) the fertilization side) were smaller for the LJ43 strain than those for LY002. The ratio of secondary to primary roots was much higher in LJ-far than that in LY-far. The N contents of leaves, primary roots and secondary roots were higher in LJ-far than those in LY-far. The leaf distribution of total N and most FAAs were higher in LJ-far than those in LY-far. On lines far from the fertilization sides, gamma-aminobutyric acid and alanine tended to accumulate more in LY002 than in LJ43, while glutamic acid and theanine tended to accumulate more in LJ43 than in LY002. Indeed, there were some genes (CsGABA-T, CsGAD1, CsGAD2, CsAlaAT and CsGs(TS)1) involved in the conversion of these amino acids. Moreover, the expression of some genes involved in N transport and assimilation (such as CsNRT2.5 and CsGS2) were up regulated in LJ-far, which may also help improve the low N tolerance in LJ43. In summary, the greater adaptive ability of LJ43 might be related to: (1) preferential development of secondary roots; (2) better N transporting ability; (3) key gene regulation to FAA transformation. Our findings may contribute to the advancement of tea planting patterns and a more complete understanding of the physiological and molecular mechanisms of tea responses to the spatial heterogeneity of N.

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