Transcriptomic and Expression Analysis of the Salivary Glands in Brown Planthoppers, Nilaparvata lugens (Hemiptera: Delphacidae)

文献类型： 外文期刊

第一作者： Jia, Haokang

作者： Jia, Haokang;Li, Zhen;Liu, Yudi;Hou, Maolin

作者机构：

关键词： Nilaparvata lugens; transcriptome; salivary gland

期刊名称：JOURNAL OF ECONOMIC ENTOMOLOGY （ 影响因子：2.381； 五年影响因子：2.568 ）

ISSN： 0022-0493

年卷期： 2018 年 111 卷 6 期

页码：

收录情况： SCI

摘要： The brown planthopper (BPH), Nilaparvata lugens Stal (Hemiptera: Delphacidae), is a serious rice pest because of its destructive feeding. The salivary glands of the BPH play a key role in successful feeding. In this study, we explored the salivary gland transcriptome (sialotranscriptome) of adult BPHs using Illumina sequencing and a total of 55,913 transcripts and 45,421 unigenes were obtained. We identified one reference gene RPL9 (Ribosomal protein L9) and 19 salivary protein genes from the BPH sialotranscripome, which were categorized as those involved in sugar metabolism, extra-oral digestion of cell wall components, detoxification, and suppression of plant defenses. Tissue expression profiles of 19 salivary protein genes analysis revealed that the expression level of alpha-glucosidase family 31 had no difference in five tissues, suggesting that it may have functions in the whole-body parts. Glucose dehydrogenase (flavine adenine dinucleotide, quinone)-like was expressed highly in the salivary gland, which might play putative role in insect feeding. Glucose dehydrogenase (acceptor) was expressed the highest level in head without salivary gland. Other salivary protein genes were highly expressed in gut or malpighian tubule, suggesting that they may play roles in digestive and detoxification mechanism. Moreover, we detected RPL9 is one of the genes that is most consistently present for acquisition of gene expression in different tissues. Thus, RPL9 can be a new reference for expression studies of BPH. The obtained BPH sialotranscripome provides a list of genes that have potential roles in feeding and interaction between BPHs and rice plants.

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