Effect of Weakly Basic Conditions on the Separation and Purification of Flavonoids and Glycosides from Chrysanthemum morifolium Tea

文献类型： 外文期刊

第一作者： Wang, Yuxiao

作者： Wang, Yuxiao;Wu, Yue;Li, Mo;Pang, Sicheng;Liang, Zhe;Ni, Yuanying;Xu, Zhenzhen;Wang, Yuxiao;Wu, Yue;Li, Mo;Pang, Sicheng;Liang, Zhe;Ni, Yuanying;Wang, Yuxiao;Wu, Yue;Li, Mo;Pang, Sicheng;Liang, Zhe;Ni, Yuanying

作者机构：

关键词： weakly basic condition; Chrysanthemum morifolium tea; chlorogenic acid; apigenin-7-O-glucoside; hydrolysis

期刊名称：MOLECULES （ 影响因子：4.411； 五年影响因子：4.587 ）

ISSN： 1420-3049

年卷期： 2019 年 24 卷 2 期

页码：

收录情况： SCI

摘要： Tea brewed from chrysanthemum flowers has been widely used in Chinese medicine. The possibly medicinal compounds in Chrysanthemum morifolium tea can be purified by preparative high performance liquid chromatography (HPLC), but this is usually done with acidic conditions, which leads to the hydrolysis of glycosides. In hopes of avoiding this hydrolysis, we explored the effect of weakly basic conditions on the separation and purification of flavonoids and glycosides from Chrysanthemum morifolium. We also explored the effects of weakly basic conditions on chlorogenic acid (3-CQA) and apigenin-7-O-glucoside (A7G). Our results show that the concentration of the weakly basic ammonium hydrogen carbonate and time had no significant effect on A7G, p < 0.01, but it had a significant effect on 3-CQA, p < 0.01. HPLC and ultraviolet (UV) analysis showed that the structure of 3-CQA is destroyed in weakly basic conditions. Caffeic acid, quinic acid, and 3,4-dihydroxymandelic acid, which is a hydrolysate of 3-CQA, were identified by ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS). The results showed that weakly basic conditions could be used for the purification of flavonoids and glycosides but not for caffeoylquinic acids. Moreover, our work clarified the hydrolysis behaviour of caffeoylquinic acids, which can be helpful for research into their functional aspects.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序