Sequence analysis and characterization of type I interferon and type II interferon from the critically endangered sturgeon species, A. dabryanus and A. sinensis

文献类型： 外文期刊

第一作者： Xu, Qiaoqing

作者： Xu, Qiaoqing;Luo, Kai;Gao, Weihua;Zhang, Wenbing;Xu, Qiaoqing;Gao, Weihua;Xu, Qiaoqing;Gao, Weihua;Zhang, Shuhuan;Wei, Qiwei;Luo, Kai;Zhang, Wenbing

作者机构：

关键词： Interferon; Chinese sturgeon; Dabry's sturgeon; Antiviral and bacterial response

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.581； 五年影响因子：4.851 ）

ISSN： 1050-4648

年卷期： 2019 年 84 卷

页码：

收录情况： SCI

摘要： In the present study, we identify three type I interferon (IFN) genes (Ad/AsIFNe1-3) and a type II IFN gene (Ad/AsIFN gamma) from the Dabry's sturgeon (Acipenser dabryanus) and the Chinese sturgeon (Acipenser sinensis). Sequence analysis revealed that Ad/AsIFNe1-3 and Ad/AsIFN gamma contain several conserved characteristics, including signal peptides, interferon alpha, beta, and delta (IFabd) domains, and N-glycosylation sites. Ad/AsIFNe1-3 belongs to the type I IFN group I subgroup, possessing two conserved cysteines residues (Cl and C3), and Ad/AsIFN gamma contained a conserved nuclear localization sequence (NLS) motif. Ad/AsIFNe1-3 and Ad/AsIFN gamma contain signature motifs indicative of their corresponding IFN group. The Ad/AsIFNe1-3 and Ad/AsIFN gamma genes were found to consist of 5 exons/4 introns and 4 exons/3 introns, respectively. These IFNs were separated by four phase 0 introns (type I IFN) and three phase 0 introns (type II IFN). The sequences of IFNe1-3 and IFN gamma from the Dabry's sturgeon and the Chinese sturgeon were closely aligned, suggested that these two species are closely related. Phylogenetic analysis revealed that Ad/AsIFNe1-3 and Ad/AsIFN gamma clustered together with the corresponding homologous proteins from other fish species. AdIFNe1-3 were found to be high expressed in early embryonic development, suggesting that AdIFNe1-3 might indicate maternal transmission, while AdIFN gamma may not mediate embryonic development. Tissue distribution analysis revealed that MIFNe1-3 and AdIFN gamma carry out biological functions in immune and non -immune tissues compartments. AdIFNe1-3 and AdIFN gamma can be stimulated by polyinosinic-polycytidylic acid (poly I:C) and lipopolysaccharides (LPS). AdIFNe1-3 have stronger antiviral activity than AdIFN gamma, and AdIFN gamma has a stronger antibacterial activity than AcilFNe1-3. The differential responses of these genes to poly I:C and LPS suggest differences in the mechanisms of defense against viruses and bacteria.

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