A comparison of methods for effective differentiation of the frozen-thawed 3T3-L1 cells

文献类型： 外文期刊

第一作者： Zhao, Xueyan

作者： Zhao, Xueyan;Hu, Hongmei;Wang, Cheng;Bai, Liya;Wang, Yanping;Wang, Jiying;Wang, Weiwei

作者机构：

关键词： 3T3-L1 preadipocytes; Adipogenic cocktail; Rosiglitazone; 3-Isobutyl-1-methylxanthine

期刊名称：ANALYTICAL BIOCHEMISTRY （ 影响因子：3.365； 五年影响因子：3.049 ）

ISSN： 0003-2697

年卷期： 2019 年 568 卷

页码：

收录情况： SCI

摘要： The differentiation efficiency of 3T3-L1 preadipocytes is an essential factor affecting studies on cellular mechanisms associated with obesity, diabetes, and related disorders. Differentiation of 3T3-L1 cells is commonly induced by an adipogenic cocktail containing insulin, dexamethasone (DEX), and 3-isobutyl-1-methylxanthine (IBMX). However, 3T3-L1 cells after freezing and thawing for many times always have a low differentiation efficiency. To solve this problem, we compared the differentiation efficiency of six commonly used adipogenic cocktails and protocols published in 2017. On this basis, we further compared 18 adipogenic cocktails with 2 mu M rosiglitazone added and/or with a prolonged treatment with IBMX. The results revealed that the adipogenic cocktail containing 0.5 mM IBMX, 1 mu M DEX, and 10 mu g/mL insulin was the most effective for the frozen-thawed 3T3-L1 cells differentiation. Rosiglitazone, and IBMX under a prolonged treatment, could improve the differentiation efficiency of the frozen-thawed 3T3-L1 cells. However, the effect was closely related to concentrations of agents in the adipogenic cocktails.

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