文献类型： 外文期刊
第一作者： Wang, Xuemin
作者： Wang, Xuemin;Gao, Yuhao;Cui, Miaomiao;Gao, Yuhao;Li, Jindong;Huang, Jianping;Cao, Jiangbo;Ban, Liping;Gao, Yuhao;Chen, Zhihong
作者机构：
关键词： aphid; behavior assay; (E)-beta-farnesene synthase gene; gas chromatography mass spectrometry; Medicago sativa
期刊名称：PEST MANAGEMENT SCIENCE （ 影响因子：4.845； 五年影响因子：4.674 ）
ISSN： 1526-498X
年卷期： 2019 年 75 卷 3 期
页码：
收录情况： SCI
摘要： BACKGROUND Pea aphid (Acyrthosiphon pisum Harris) is one of the major pests in alfalfa crops, causing significant yield losses. (E)-beta-farnesene (E beta F), an alarm pheromone released by pea aphid, is generic to many species of aphids, and is used to minimize potential danger from predators and parasitoids by avoiding the source of the pheromone. RESULTS In this study, E beta F synthase gene was constructed into a plant expression vector, and overexpressed in alfalfa (Medicago sativa L.), with expression among transgenic lines confirmed by qRT-PCR. Subcellular localization analysis showed that E beta F synthase gene was expressed in the plasma membrane and nucleus of the leaf. GC/MS of extraction from transgenic alfalfa indicated emission of E beta F ranging from 5.92 to 13.09 ng day(-1) g(-1) fresh tissue. Behavior assays in Y-olfactometers demonstrated that transgenic alfalfa expressing AaE beta F gene could repel pea aphids, with aphids taking a significantly longer time to select a transgenic line compared with the control line (P < 0.01). CONCLUSION We have demonstrated a potentially valuable strategy of using E beta F synthase genes for aphid control in alfalfa. (c) 2018 Society of Chemical Industry
分类号：
- 相关文献
作者其他论文 更多>>
-
Chromosome-level genome assembly of Odontothrips loti Haliday (Thysanoptera: Thripidae)
作者：Luo, Yingning;Dai, Wenting;Miao, Miao;Ban, Liping;Wei, Shuhua;Wang, Ying;Zhang, Rong
关键词：
-
Nonspecific phospholipases C3 and C4 interact with PIN-FORMED2 to regulate growth and tropic responses in Arabidopsis
作者：Li, Teng;Zhang, Shujuan;Li, Xuebing;Yuan, Jingya;Zhang, Wenhua;Zhang, Qun;Yao, Shuaibing;Wang, Xuemin;Yao, Shuaibing;Wang, Xuemin;Jia, Qianru
关键词：
-
Phosphorylated transcription factor PuHB40 mediates ROS-dependent anthocyanin biosynthesis in pear exposed to high light
作者：Zhang, Lu;Wang, Lu;Fang, Yongchen;Gao, Yuhao;Yang, Shulin;Ni, Junbei;Teng, Yuanwen;Bai, Songling;Su, Jun;Teng, Yuanwen
关键词：
-
Method validation, dissipation and risk ranking of multi-pesticides in blueberry
作者：Pan, Wei;Pei, Tao;Wang, Fuyun;Li, Li;Lv, Ying;Pei, Tao;Li, Jindong;Qin, Shu;Li, Jindong;Li, Li
关键词：Blueberry; Pesticide; QuEChERS; Dissipation; Risk ranking
-
Beta diversity of grasshoppers and predatory beetles across steppes is closely associated with altitude and average annual precipitation in Ningxia, northwest China
作者：Zhang, Haixiang;Cao, Ziyu;Wang, Ying;Zhang, Rong;Wei, Shuhua;Zhang, Haixiang;Ban, Liping;Shi, Xiaoyu;Cheng, Rui;Cao, Ziyu
关键词：Ningxia Steppe; Grasshopper; Predatory beetle; Beta diversity; Environmental factors
-
Genome-wide analysis and expression of the aquaporin gene family in Avena sativa L.
作者：Zhou, Xinyue;Yi, Dengxia;Ma, Lin;Wang, Xuemin
关键词：Avena sativa L.; aquaporin; gene family; genome-wide; abiotic stress
-
Accurately estimate soybean growth stages from UAV imagery by accounting for spatial heterogeneity and climate factors across multiple environments
作者：Che, Yingpu;Gu, Yongzhe;Bai, Dong;Li, Delin;Li, Jindong;Li, Ying-hui;Jin, Xiuliang;Qiu, Li-juan;Che, Yingpu;Li, Jindong;Li, Ying-hui;Jin, Xiuliang;Qiu, Li-juan;Che, Yingpu;Zhao, Chaosen;Wang, Ruizhen;Wang, Qiang;Qiu, Hongmei;Huang, Wen;Yang, Chunyan;Zhao, Qingsong;Liu, Like;Wang, Xing;Xing, Guangnan;Hu, Guoyu;Shan, Zhihui
关键词：Soybean growth stages; Multi-environment trials; Photothermal accumulation area; Spatial heterogeneity; Unmanned aircraft vehicle
