Improved antibacterial effects of alkali-transformed saponin from quinoa husks against halitosis-related bacteria

文献类型： 外文期刊

第一作者： Sun, Xiaoyan

作者： Sun, Xiaoyan;Zhang, Zhiguo;Sun, Xiaoyan;Yang, Xiushi;Xue, Peng;Ren, Guixing

作者机构：

关键词： Quinoa; Halitosis; Alkali-transformation; Antibacterial activity; Antibacterial mechanism

期刊名称：BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE （ 影响因子：3.659； 五年影响因子：3.767 ）

ISSN： 1472-6882

年卷期： 2019 年 19 卷

页码：

收录情况： SCI

摘要： Background: Quinoa is a food crop native to the Andes. The process of dehulling quinoa can produce approximately 8-12% husk, which is often discarded because it contains bitter saponin. Saponin derived from quinoa has been reported to exhibit anti-inflammatory and antifungal activity. However, the antibacterial effects of quinoa saponin against halitosis-related bacteria are still unclear. Methods: In this study, quinoa saponin (QS) and alkali-transformed saponin (ATS) were separated by AB-2 resin to obtain QS-30, QS-80, ATS-30 and ATS-80. Halitosis-related bacteria included Porphyromonas gingivalis (P. gingivalis), Clostridium perfringens (C. perfringens) and Fusobacterium nucleatum (F. nucleatum). The MIC and MBC were determined using gradient dilutions in 96-well plates, and the saponins were identified by HPLC and mass spectrometry. The changes in membrane integrity were tested using a microplate reader, the membrane potential was tested by spectrofluorometry, and the morphological characteristics were examined using a transmission electron microscope to explore the antibacterial mechanisms. Results: Antibacterial assays indicated that QS-80 and ATS-80 showed inhibitory activity. In addition, ATS-80 exerted a stronger inhibitory effect than QS-80, especially against Fusobacterium nucleatum, with a lower minimum inhibitory concentration (31.3g/mL) and a lower minimum bactericidal concentration (125g/mL). ATS-80 destroyed the bacterial membrane structure, leading to bacterial death. Conclusions: Based on the excellent antibacterial activity and economic prospects of quinoa husk, ATS-80 could be used as an antibacterial agent to treat halitosis.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序