Preparation of Immobilized Lipase Based on Hollow Mesoporous Silica Spheres and Its Application in Ester Synthesis

文献类型： 外文期刊

第一作者： Dong, Zhe

作者： Dong, Zhe;Shi, Jie;Zheng, Ming-Ming;Huang, Feng-Hong;Jiang, Meng-Ying;Jiang, Meng-Ying

作者机构：

关键词： immobilized lipase; hydrophobic modification; enzymatic esterification; phytosterols esters; response surface methodology

期刊名称：MOLECULES （ 影响因子：4.411； 五年影响因子：4.587 ）

ISSN： 1420-3049

年卷期： 2019 年 24 卷 3 期

页码：

收录情况： SCI

摘要： In this study, Candida rugosa lipase (CRL) was immobilized into modified hollow mesoporous silica (HMSS) materials with different hydrophobicity. Among propyl-(C-3), phenyl-(C-6), octyl-(C-8), and octadecyl-(C-18) modified HMSS as well as native HMSS, taking advantage of more hydrophobic microenvironment, the HMSS-C-18-CRL showed exceptional performance in enzymatic esterification reaction. Using the novel HMSS-C-18 with immobilized CRL (HMSS-C-18-CRL), we investigated the esterification of phytosterols with polyunsaturated fat acid (PUFA) in a solvent-free system for the production of phytosterols esters. Response surface methodology (RSM) was applied to model and optimize the reaction conditions, namely, the enzyme load (5-25%), reaction time (10-110 min), molar ratio of -linolenic acid (ALA)/phytosterols (1:1-7:1) and represented by the letters E, T, and M respectively. Best-fitting models were successfully established by multiple regressions with backward elimination. The optimum production was achieved at 70 min for reaction time, 20% based on the weight of substrate for enzyme loading, and 5.6:1 for ALA/phytosterols molar ratio. Under optimized conditions, a conversion of about 90 +/- 2% was achieved. These results indicated that HMSS-C-18-CRL demonstrates to be a promising catalyst and can be potentially applied in the functional lipid production.

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