Three-Phase Partitioning for the Extraction and Purification of Polysaccharides from the Immunomodulatory Medicinal Mushroom Inonotus obliquus

文献类型： 外文期刊

第一作者： Liu, Zhendong

作者： Liu, Zhendong;Li, Liang;Chen, Jiafu;Yu, Dongsheng;Liu, Xiaoxiao;Zhang, Henan;Lin, Chi-Chung;Wang, Wenhan;Jia, Wei;Yu, Dongsheng;Liu, Xiaoxiao;Zhang, Henan;Lin, Chi-Chung;Wang, Wenhan;Jia, Wei;Yu, Dongsheng;Liu, Xiaoxiao;Sun, Wenbo;Chen, Zhi

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关键词： Inonotus obliquus; three-phase partitioning; free-radical scavenging abilities; antioxidant activity; immunological activity

期刊名称：MOLECULES （ 影响因子：4.411； 五年影响因子：4.587 ）

ISSN： 1420-3049

年卷期： 2019 年 24 卷 3 期

页码：

收录情况： SCI

摘要： Polysaccharides from the immunomodulatory medicinal mushroom Inonotus obliquus (IOPS) were extracted and purified using three-phase partitioning (TPP), which is an efficient, fast, safe, and green purification technique. An optimal extraction procedure that gave a good 2.2% isolated yield was identified, using the following protocol: a solid-liquid ratio of 1 g to 12 mL; mass fraction of (NH4)(2)SO4 20% (w/v); 11 mL t-butanol; pH 8.0; temperature 30 degrees C; and extraction time 30 min. The purified IOPS was shown to be a proteoglycan of 40 kDa molecular weight comprising of d-galactose, d-glucose, d-xylose, and d-mannose in a molar ratio of 2.0:3.5:1.0:1.5. The purified IOPS displayed strong free-radical scavenging abilities, antioxidant activities, and immunological activity in vitro. IOPS' Trolox antioxidant equivalent capacity and ferric-reducing ability of plasma were 251.2 mu mol Trolox/g sample and 1040.5 mu mol Fe2+/g sample, respectively, with the activity of its immunomodulatory behavior shown to be gradient dependent.

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