Protection of Porcine Intestinal-Epithelial Cells from Deoxynivalenol-Induced Damage by Resveratrol via the Nrf2 Signaling Pathway

文献类型： 外文期刊

第一作者： Yang, Jun

作者： Yang, Jun;Yang, Jun;Zhu, Cui;Ye, Jinling;Lv, Yantao;Chen, Zhuang;Jiang, Zongyong;Wang, Li;Jiang, Zongyong

作者机构：

关键词： resveratrol; deoxynivalenol; IPEC-J2 cells; oxidative damage; Nrf2 signaling pathway

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：5.279； 五年影响因子：5.269 ）

ISSN： 0021-8561

年卷期： 2019 年 67 卷 6 期

页码：

收录情况： SCI

摘要： Deoxynivalenol (DON), a common mycotoxin, usually induces oxidative stress and affects the intestinal health of humans and animals. This study investigated the protective effect of resveratrol (RES), a natural antioxidant, on alleviating the cytotoxicity induced by DON in the porcine intestinal-epithelial cell line (IPEC-J2). Cells were incubated with RES for 24 h and then exposed to DON for another 24 h. Cell viability, proliferation, apoptosis, and oxidative-stress indicators were determined. In comparison with DON-only-treated cells, pretreatment with RES (15 mu M) increased the cell viability (79.74 +/- 2.02 vs 90.98 +/- 2.66%, P < 0.01), improved proliferation (EdU-positive cells, 26.42 +/- 1.12 vs 32.05 +/- 0.78%, P < 0.01), decreased accumulation of intracellular reactive oxygen species (ROS, 1.68 +/- 0.05 vs 1.29 +/- 0.06, P < 0.01), stabilized mitochondrial-membrane potential (MMP, 8.98 +/- 1.40 vs 2.29 +/- 0.76, P < 0.001), and prevented apoptosis induced by DON (13.91 +/- 1.20 vs 6.83 +/- 0.52%, P < 0.01). RES activated the Nrf2 signaling pathway, and transfection with Nrf2 siRNA abrogated the protection of RES against DON-induced cytotoxicity, accumulation of intracellular ROS, and mitochondria dependent apoptosis. Collectively, RES protects IPEC-J2 cells against DON-induced damage at least partly via the Nrf2 signaling pathway.

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