Citrus sinensis MYB Transcription Factor CsMYB85 Induce Fruit Juice Sac Lignification Through Interaction With Other CsMYB Transcription Factors

文献类型： 外文期刊

第一作者： Jia, Ning

作者： Jia, Ning;Sun, Yufeng;Liu, Jiameng;Sun, Jing;Huang, Yatao;Lu, Jia;Jin, Nuo;Li, Minmin;Fan, Bei;Jia, Ning;Sun, Yufeng;Liu, Jiameng;Sun, Jing;Huang, Yatao;Lu, Jia;Jin, Nuo;Li, Minmin;Fan, Bei;Jia, Ning;Lv, Yuemeng;Imam, Khandaker Md Sharif Uddin;Xin, Fengjiao;Liu, Jiqin;Tan, Penghui

作者机构：

关键词： Citrus sinensis; CsMYB85; juice sacs; lignification; postharvest

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：5.753； 五年影响因子：6.612 ）

ISSN： 1664-462X

年卷期： 2019 年 10 卷

页码：

收录情况： SCI

摘要： Varieties of Citrus are commercially important fruits that are cultivated worldwide and are valued for being highly nutritious and having an appealing flavor. Lignification of citrus fruit juice sacs is a serious physiological disorder that occurs during postharvest storage, for which the underlying transcriptional regulatory mechanisms remain unclear. In this study, we identified and isolated a candidate MYB transcription factor, CsMYB85, that is involved in the regulation of lignin biosynthesis in Citrus sinensis, which has homologs in Arabidopsis and other plants. We found that during juice sac lignification, CsMYB85 expression levels increase significantly, and therefore, suspected that this gene may control lignin biosynthesis during the lignification process. Our results indicated that CsMYB85 binds the CsMYB330 promoter, regulates its expression, and interacts with CsMYB308 in transgenic yeast and tobacco. A transient expression assay indicated that Cs4CL1 expression levels and lignin content significantly increased in fruit juice sacs overexpressing CsMYB85. At4CL1 expression levels and lignin content were also significantly increased in Arabidopsis overexpressing CsMYB85. We accordingly present convincing evidence for the participation of the CsMYB85 transcription factor in fruit juice sac lignification, and thereby provide new insights into the transcriptional regulation of this process in citrus fruits.

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