Overcoming Cabbage Crossing Incompatibility by the Development and Application of Self-Compatibility-QTL-Specific Markers and Genome-Wide Background Analysis

文献类型： 外文期刊

第一作者： Xiao, Zhiliang

作者： Xiao, Zhiliang;Han, Fengqing;Hu, Yang;Xue, Yuqian;Fang, Zhiyuan;Yang, Limei;Zhang, Yangyong;Liu, Yumei;Li, Zhansheng;Wang, Yong;Zhuang, Mu;Lv, Honghao

作者机构：

关键词： cabbage; crossing incompatibility; quantitative trait loci; genetic mapping; genomic background analysis

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：5.753； 五年影响因子：6.612 ）

ISSN： 1664-462X

年卷期： 2019 年 10 卷

页码：

收录情况： SCI

摘要： Cabbage hybrids, which clearly present heterosis vigor, are widely used in agricultural production. We compared two S5 haplotype (Class II) cabbage inbred-lines 87-534 and 94-182: the former is highly SC while the latter is highly SI; sequence analysis of SI-related genes including SCR, SRK, ARC1, THL1, and MLPK indicates the some SNPs in ARC1 and SRK of 87-534; semi-quantitative analysis indicated that the SI-related genes were transcribed normally from DNA to mRNA. To unravel the genetic basis of SC, we performed whole-genome mapping of the quantitative trait loci (QTLs) governing self-compatibility using an F-2 population derived from 87-534 x 96-100. Eight QTLs were detected, and high contribution rates (CRs) were observed for three QTLs: qSC7.2 (54.8%), qSC9.1 (14.1%) and qSC5.1 (11.2%). 06-88 (CB201 x 96-100) yielded an excellent hybrid. However, F-1 seeds cannot be produced at the anthesis stage because the parents share the same S-haplotype (S57, class I). To overcome crossing incompatibility, we performed rapid introgression of the self-compatibility trait from 87-534 to 96-100 using two self-compatibility-QTL-specific markers, BoID0709 and BoID0992, as well as 36 genome-wide markers that were evenly distributed along nine chromosomes for background analysis in recurrent back-crossing (BC). The transfer process showed that the proportion of recurrent parent genome (PRPG) in BC4F1 was greater than 94%, and the ratio of individual SC plants in BC4F1 reached 100%. The newly created line, which was designated SC96-100 and exhibited both agronomic traits that were similar to those of 96-100 and a compatibility index (CI) greater than 5.0, was successfully used in the production of the commercial hybrid 06-88. The study herein provides new insight into the genetic basis of self-compatibility in cabbage and facilitates cabbage breeding using SC lines in the male-sterile (MS) system.

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