Oxidative Stress Disrupts Gill Function in Eriocheir sinensis: Consequences for Ion Transport, Apoptosis, and Autophagy
文献类型： 外文期刊
第一作者： Feng, Wenrong
作者： Feng, Wenrong;He, Qinghong;Xu, Yuanfeng;Jiang, Gang;Li, Jianlin;Jia, Rui;Tang, Yongkai;Feng, Wenrong;Jia, Rui;Tang, Yongkai;Yang, Qiqin;Zhou, Jun
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期刊名称：ANTIOXIDANTS （ 影响因子：6.6； 五年影响因子：7.3 ）
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年卷期： 2025 年 14 卷 8 期
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收录情况： SCI
摘要： Oxidative stress is a key mediator of physiological dysfunction in aquatic organisms under environmental challenges, yet its comprehensive impacts on gill physiology require further clarification. This study investigated the molecular and cellular responses of Eriocheir sinensis gills to hydrogen peroxide (H2O2)-induced oxidative stress, integrating antioxidant defense, ion transport regulation, and stress-induced cell apoptosis and autophagy. Morphological alterations in the gill filaments were observed, characterized by septum degeneration, accumulation of haemolymph cells, and pronounced swelling. For antioxidant enzymes like catalase (CAT) and glutathione peroxidase (GPx), activities were enhanced, while superoxide dismutase (SOD) activity was reduced following 48 h of exposure. Overall, the total antioxidant capacity (T-AOC) showed a significant increase. The elevated concentrations of malondialdehyde (MDA) and H2O2 indicated oxidative stress. Ion transport genes displayed distinct transcription patterns: Na+-K+-2Cl- co-transporter-1 (NKCC1), Na+/H+ exchanger 3 (NHE3), aquaporin 7 (AQP7), and chloride channel protein 2 (CLC2) were significantly upregulated; the alpha-subunit of Na+/K+-ATPase (NKA alpha) and carbonic anhydrase (CA) displayed an initial increase followed by decline; whereas vacuolar-type ATPase (VATP) consistently decreased, suggesting compensatory mechanisms to maintain osmotic balance. Concurrently, H2O2 triggered apoptosis (Bcl2, Caspase-3/8) and autophagy (beclin-1, ATG7), likely mediated by MAPK and AMPK signaling pathways. These findings reveal a coordinated yet adaptive response of crab gills to oxidative stress, providing new insights into the mechanistic basis of environmental stress tolerance in crustaceans.
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