Enhancing pest control in pear orchards: Harnessing nanocarrier SPc for efficient delivery of emamectin benzoate and dslgl to safeguard beneficial insects

文献类型： 外文期刊

第一作者： Li, Jianying

作者： Li, Jianying;Zhang, Dongyue;Wang, Yilin;Li, Zhen;Liu, Xiaoxia;Zhang, Songdou;Li, Jianying;Zhang, Dongyue;Wang, Yilin;Li, Zhen;Liu, Xiaoxia;Zhang, Songdou;Xing, Kun;Zhao, Fei;Zhang, Songdou

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关键词： Insecticide; Lethal giant larvae (lgl) gene; Grapholitha molesta; Cacopsylla chinensis; biopesticide

期刊名称：ENTOMOLOGIA GENERALIS （ 影响因子：4.6； 五年影响因子：4.4 ）

ISSN： 0171-8177

年卷期： 2025 年 45 卷 1 期

页码：

收录情况： SCI

摘要： Chemical control stands as a cornerstone in orchard pest management, yet concerns arise from prolonged usage including resistance development, non-target organism risks, and environmental repercussions. Recent advancements leveraging nanocarriers for co-delivery of insecticides and dsRNA present a promising, efficient, and environmentally sound approach to pest control. In this study, we harnessed SPc for the simultaneous delivery of emamectin benzoate (EB) and dsRNA targeting lethal giant larvae (lgl) gene to combat two major orchard pests, Grapholitha molesta and Cacopsylla chinensis. This innovative strategy not only bolstered insecticide efficacy but also addressed resistance challenges and safety considerations. Initially, we assessed the efficacy of EB across various life stages of the pests. Subsequent RNA-seq analysis unveiled lgl gene upregulation following EB treatment, with lgl gene silencing heightening pest susceptibility to EB. By utilizing the pET30a-BL21-RNase III- system to express hairpin RNA hplgl, we engineered a ternary biopesticide (hplgl-SPc-EB complex). This biopesticide exhibited remarkable control over G. molesta and C. chinensis, achieving substantial mortality rates at minimal EB doses. Notably, the ternary biopesticide posed no detrimental impacts on natural enemies, enabling seamless integration with biological control methods. Our findings highlight the advantages of ternary biopesticides in reducing insecticide reliance, promoting environmental sustainability, managing resistance, and offering a comprehensive strategy for orchard pest management.

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