The CpxA/CpxR Two-Component System Affects Biofilm Formation and Virulence in Actinobacillus pleuropneumoniae

文献类型： 外文期刊

第一作者： Li, Huan

作者： Li, Huan;Liu, Feng;Peng, Wei;Van, Kang;Zhao, Haixu;Liu, Ting;Cheng, Hui;Chang, Peixi;Chen, Huanchun;Bei, Weicheng;Li, Huan;Liu, Feng;Peng, Wei;Van, Kang;Zhao, Haixu;Liu, Ting;Cheng, Hui;Chang, Peixi;Chen, Huanchun;Bei, Weicheng;Li, Huan;Liu, Feng;Peng, Wei;Van, Kang;Zhao, Haixu;Liu, Ting;Cheng, Hui;Chang, Peixi;Chen, Huanchun;Bei, Weicheng;Yuan, Fangyan

作者机构：

关键词： Actinobacillus pleuropneumoniae; CpxA/CpxR; biofilm; rpoE; virulence

期刊名称：FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY （ 影响因子：5.293； 五年影响因子：5.882 ）

ISSN： 2235-2988

年卷期： 2018 年 8 卷

页码：

收录情况： SCI

摘要： Gram-negative bacteria have evolved numerous two-component systems (TCSs) to cope with external environmental changes. The CpxA/CpxR TCS consisting of the kinase CpxA and the regulator CpxR, is known to be involved in the biofilm formation and virulence of Escherichia coli. However, the role of CpxA/CpxR remained unclear in Actinobacillus pleuropneumoniae, a bacterial pathogen that can cause porcine contagious pleuropneumonia (PCP). In this report, we show that CpxA/CpxR contributes to the biofilm formation ability of A. pleuropneumoniae. Furthermore, we demonstrate that CpxA/CpxR plays an important role in the expression of several biofilm-related genes in A. pleuropneumoniae, such as rpoE and pgaC. Furthermore, The results of electrophoretic mobility shift assays (EMSAs) and DNase I footprinting analysis demonstrate that CpxR-P can regulate the expression of the pgaABCD operon through rpoE. In an experimental infection of mice, the animals infected with a cpxA/cpxR mutant exhibited delayed mortality and lower bacterial loads in the lung than those infected with the wildtype bacteria. In conclusion, these results indicate that the CpxA/CpxR TCS plays a contributing role in the biofilm formation and virulence of A. pleuropneumoniae.

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